不同寄主红花寄生叶提取物抗氧化能力的研究  

Study of Antioxidant Capacity of Leaf Extracts of Scurrula parasitica from Different Host Trees

作　　者：陈莺莺[1] 陈炳华[1] 赖京菁[1] 周冰洁[1] 连俊蕊[1] 

机构地区：[1]福建师范大学生命科学学院,福建福州350108

出　　处：《福建师范大学学报（自然科学版）》2011年第2期94-100,共7页Journal of Fujian Normal University：Natural Science Edition

基　　金：福建省科技厅资助项目(2008F5018);福建师范大学国家级生物学实验教学示范中心创新性研究项目(2009ls007);福建师范大学大学生课外科技计划项目(BKL2009081)

摘　　要：采用FRAP法和TEAC法测定了6种寄主植物(女贞、石榴、荷花玉兰、长梗柳、桑、夹竹桃)来源的红花寄生叶提取物抗氧化能力,并采用RP-HPLC检测了提取物中槲皮素和山奈酚质量分数.结果表明:红花寄生叶提取物具有较强的抗氧化能力,且活性强弱受不同提取剂和寄主植物的影响显著(p<0.05).在3种提取剂中,以体积分数为80%丙酮获得的提取物抗氧化能力最强,其cFRAP值和cTEAC分别为1.07mmol.g-1和8.32 mmol.g-1;在6种寄主植物中,以寄生于长梗柳、石榴、桑树上的红花寄生抗氧化能力较强.此外,统计分析显示红花寄生叶提取物抗氧化能力与总酚质量分数呈显著正相关(R2≥0.70),但与黄酮质量分数不呈明显相关关系(R2<0.30).可见,大分子质量的酚类物质是红花寄生抗氧化活性的主要贡献者.The antioxidant capacity of leaf extracts from Scurrula parasitica,parasitic on Ligustrum lucidum,Punica granatum,Magnolia grandiflora,Salix dunnii,Morus alba,Nerium indicum,were detected by FRAP assay and TEAC assay. The content of quercetin and kaempferol of leaf extracts were measured by reverse-phase high-performance liquid chromatography（RP-HPLC）.The result showed that leaf extracts of S.parasitica exhibited strong antioxidant capacity,which was significantly affected by different extraction agents and host trees.Among the three extraction solvents,extracts of 80% acetone had the highest antioxidant activity with the cFRAP and cTEAC values of 1.08 and 8.32 mmol·g-1,respectively.Among the six different host trees,extracts sourced from Salix matsudana,Punica granatum and Morus alba showed higher antioxidant capacity.In addition,the antioxidant activity of leaf extracts of S.parasitica might be attributed to the total phenolic content（R2≥0.70）,but not flavonoid content（R20.30）.Thus,high molecular phenolics might be a major contributor of antioxidant capacity of S.parasitica.

关 键 词：红花寄生 FRAP法 TEAC法 抗氧化能力 寄主植物 

分 类 号：Q946.91[生物学—植物学] S792.95[农业科学—林木遗传育种]