顺铂对原代培养兔近端肾小管上皮细胞酶活力的影响以及牛磺酸、三七皂苷和茵陈素的保护作用  

Influence of cisplatin on activities of enzymes in primary culture of rabbit proximal tubular cells,and protective effects of taurine,panax notoginseng saponins and scoparoneon

作　　者：刘世杰[1] 周世文[1] 

机构地区：[1]第三军医大学新桥医院临床药理基地,重庆400037

出　　处：《中国药理学通报》1999年第5期452-455,共4页Chinese Pharmacological Bulletin

摘　　要：目的　研究顺铂对原代培养的近端肾小管上皮细胞（ＰＴＣ）的乳酸脱氢酶（ＬＤＨ），碱性磷酸酶（ＡＬＰ） ，Ｎ乙酰β氨基葡萄糖苷酶（ＮＡＧ） 活力的影响，探讨牛磺酸，三七皂苷，茵陈素对酶活力的保护作用。方法　在体外建立ＰＴＣ。顺铂损伤组：顺铂与ＰＴＣ共同保温２４ ｈ ；处理组：牛磺酸，三七皂苷，茵陈素提前与ＰＴＣ作用２４ ｈ，后再加入顺铂（２６ μｍｏｌ·Ｌ－ １）共同保温２４ ｈ。结果　顺铂（１３，２６，５２ ，７８，１０４ μｍｏｌ·Ｌ－ １）抑制ＬＤＨ，ＡＬＰ，和ＮＡＧ的活力（ Ｐ＜０－０１），牛磺酸（１，１０ ｇ·Ｌ－ １） 和茵陈素（４ ，８ ｍｇ·Ｌ－１） 可提高被顺铂抑制的ＬＤＨ、ＡＬＰ和ＮＡＧ 活力（ Ｐ＜ ０－０１） ，三七皂苷（１０ ，１００ ｍｇ·Ｌ－ １）可提高被顺铂抑制的ＬＤＨ 和ＮＡＧ活力（ Ｐ＜ ０－０１ ， Ｐ＜０－０５） ，但不能提高ＡＬＰ 活力。结论　顺铂抑制ＰＴＣ 的ＬＤＨ，ＡＬＰ，ＮＡＧ 活力，牛磺酸和茵陈素可提高被顺铂抑制的ＬＤＨ，ＡＬＰ，ＮＡＧ 活力，三七皂苷可提高被顺铂抑制的ＬＤＨ，ＮＡＧ 活力。AIM To study influence of cisplatin on activities of LDH, ALP, and NAG in primary culture of rabbit proximal tubular cells (PTC), and protective effects of taurine, panax notoginseng saponins(PNS), and scoparoneon on these. METHODS PTC were established iv vitro . PTC were incubated with cisplatin for 24 h in cisplatin group. After PTC were preincubated with drugs for 24 h, cisplatin(26μmol·L -1 )was added into culture and incubated for another 24 h in treatment groups. RESULTS Cisplatin(0, 13, 26, 52, 78,104 μmol·L -1 )significantly decreased activities of LDH, ALP, and NAG in PTC( P <0 01).Taurine(1,10 g·L -1 )and scoparoneon(4,8 mg·L -1 ) significantly increased cisplatin reduced activities of LDH, ALP and NAG, respectively( P <0 01). PNS (10,100 mg·L -1 )significantly increased cisplatin reduced activities of LDH and NAG( P <0 01, P <0 05), but not ALP. CONCLUSION Cisplatin reduced activities of LDH, ALP, and NAG in PTC. Taurine and scoparoneon significantly increased cisplatin reduced activities of LDH, ALP, and NAG in PTC, PNS could increased cisplatin reduced activities of LDH and NAG,but not ALP.

关 键 词：顺铂 牛磺酸 三七皂苷 茵陈素 肾小管 

分 类 号：R979.1[医药卫生—药品]