慢性缺氧对大鼠岩神经节神经元酸敏感离子通道亚型3和亚型2a表达的影响  

Effects of Chronic Hypoxia on Expression of Acid-sensing Ion Channels 3 and 2a of Neurons in Petrosal Ganglions of Rats

作　　者：王乾成[1] 陈祁[1] 杨荫[1] 赵书[1] 陈丽[1] 郑煜[1] 

机构地区：[1]四川大学华西基础医学与法医学院生理学教研室,成都610041

出　　处：《华西医学》2011年第2期170-173,共4页West China Medical Journal

基　　金：国家自然科学基金资助项目(30370530)~~

摘　　要：目的探讨慢性缺氧对大鼠岩神经节神经元酸敏感离子通道(acid-sensing ion channels,ASICs)亚型3(ASIC3)和亚型2a(ASIC2a)表达的影响。方法将12只健康成年SD大鼠随机分为正常组和缺氧组。用免疫组织化学法(PV)观察正常和慢性缺氧大鼠岩神经节神经元ASIC3和ASIC2a的表达。结果给予慢性缺氧刺激后,岩神经节ASIC3阳性表达神经元数目增多(P<0.05),灰度值降低(P<0.05);而ASIC2a阳性表达神经元数目和灰度值无明显变化(P>0.05)。结论慢性缺氧可上调大鼠岩神经节神经元ASIC3的表达,而对ASIC2a的表达无明显影响,提示ASIC3和ASIC2a可能在岩神经节对缺氧的反应中起着不同的作用。Objective To investigate the effects of chronic hypoxia on expression of acid-sensing ion channels（ASIC） 3 and ASIC2a in neurons of petrosal ganglions of rats.Methods A total of 12 SD rats were randomly assigned to control group and hypoxia group.The expressions of ASIC3 and ASIC2a of the neurons in the petrosal ganglions in the two groups were investigated with the immunohistochemical technique.Results The level of positive ASIC3 expression in the petrosal ganglions was higher in the hypoxia group than that in the control group（P〈0.05）;the difference of positive ASIC2a expression levels between the control group and the hypoxia group was not statistically significant（P〉0.05）.Conclusion Chronic hypoxia can significantly increase the expression of ASIC3,but not that of ASIC2a,of the neurons in the petrosal ganglions,suggesting their different roles in mediating a cellular response to chronic hypoxia.

关 键 词：岩神经节 慢性缺氧 酸敏感离子通道 大鼠 

分 类 号：R363[医药卫生—病理学]