胆囊良恶性病变组织中DNApkcs和Ku70的表达及其临床病理意义  

Expression of DNApkcs and Ku70 in benign and malignant lesions of gallbladder and its clinicopathological significance

作　　者：谭兴国[1] 杨竹林[1] 苗雄鹰[1] 

机构地区：[1]中南大学湘雅二医院肝胆疾病研究室,湖南长沙410011

出　　处：《中国普通外科杂志》2011年第2期163-168,共6页China Journal of General Surgery

摘　　要：目的探讨胆囊腺癌、癌旁组织、腺瘤性息肉和慢性胆囊炎组织中DNApkcs和Ku70的表达水平及其临床病理意义。方法方法免疫组化法检测108例胆囊腺癌、46例癌旁组织、15例腺瘤性息肉和35例慢性胆囊炎组织中DNApkcs和Ku70的表达,分析其与临床病理因素的关系。结果胆囊腺癌DNApkcs和Ku70表达阳性率(50.0%,51.9%)明显低于癌旁组织(82.6%,82.6%)、腺瘤性息肉(80.0%,80.0%)和慢性胆囊炎胆囊上皮(91.4%,88.6%)(P<0.05或P<0.01);高分化腺癌、淋巴结未转移及未侵犯周围组织器官者DNApkcs和Ku70表达阳性率明显高于低分化腺癌、淋巴结转移及侵犯周围组织器官者(P<0.05或P<0.01),但与胆囊腺癌其他临床病理特征无明显关系(P>0.05)。胆囊腺癌中DNApkcs与Ku70两者表达呈高度一致性(χ2=48.07,P<0.01),DNApkcs和Ku70表达阳性者术后生存期明显高于阴性表达者(P<0.05)。结论 DNApkcs和Ku70表达水平与恶性肿瘤进展、生物学行为及预后有关,高水平表达者恶性度低及生存期长,DNApkcs和Ku70可能成为一个反映胆囊腺癌预后的重要生物学标记物之一。Objective To investigate the expression of DNA dependent protein kinases catalytic subunit（DNApkcs） and Ku70 in gallbladder adenocarcinoma,peritumoral tissues,adenomatous polyp and chronic cholecystitis,and explore its clinicopathological significance.Methods EnVisionTM immunohistochemical method was used to detect the expression of DNApkcs and Ku70 in the routinely paraffin embedded sections of samples of gallbladder adenocarcinoma（n=108）,peritumoral tissues（n=46）,adenomatous polyp（n=15） and chronic cholecystitis（n=35）.Results The positive rates of DNApkcs and Ku70 were significantly lower in gallbladder adenocarcinoma（50.0%,51.9%）than in peritumoral tissues（82.6%,82.6%）and chronic cholecystitis（91.4%,88.6%）（P0.05 or P0.01）.The positive rates were significantly higher in the cases of well-differentiated adenocarcinoma,non-metastasis of lymph node and non-infilatrated regional tissues or organs than in those of low-differentiated adenocarcinoma,metastasis of lymph node and infilated regional tissues or organs（P0.05 or P0.01）.A high consistence was found between DNApkcs and Ku70 expression in gallbladder adenocarcinoma.Conclusions The expression of DNApkcs and Ku70 might be important biological markers reflecting the carcinogenesis,progression,biological behaviors and prognosis of gallbladder adenocarcinoma.The cases with positive expression of DNApkcs and Ku70 might have better prognosis.

关 键 词：胆囊肿瘤/病理学 胆囊炎 DNA依赖性蛋白激酶催化亚单位 KU70 免疫组织化学 

分 类 号：R735.8[医药卫生—肿瘤]