肝细胞癌Treg和FOXP3的表达及其临床意义  

Expression of Treg and FOXP3 in hepatocellular carcinoma and its significance

作　　者：郑启昌[1] 杨婧[1] 王慧[1] 王国梁[1] 胡青钢[1] 张进祥[2] 

机构地区：[1]华中科技大学同济医学院附属协和医院肝胆外科,武汉430022 [2]华中科技大学同济医学院附属协和医院急诊外科,430022

出　　处：《临床肿瘤学杂志》2011年第2期124-127,共4页Chinese Clinical Oncology

摘　　要：目的研究肝细胞癌(HCC)患者外周血CD4+CD25+调节性T细胞(Treg)和肝癌组织叉状头/翅膀状螺旋转录因子(FOXP3)的表达,并分析其与肝细胞癌临床病理的关系。方法采用流式细胞仪对HCC患者和健康志愿者外周血CD4+CD25+Treg细胞进行检测,免疫组化、Western blotting法检测FOXP3蛋白表达水平,并结合HCC患者临床病理特征进行分析。结果 HCC患者外周血CD4+CD25+Treg表达率为(16.76±5.53)%,较健康志愿者的(6.04±2.01)%显著增高(P<0.01)。免疫组化染色肿瘤组织FOXP3阳性细胞数为(103.95±34.31)个/mm2,高于癌旁组织的(29.61±4.65)个/mm2(P<0.05),相应肿瘤组织FOXP3蛋白表达也增高。CD4+CD25+Treg细胞水平与肿瘤多灶性有关。结论 HCC患者的外周血CD4+CD25+Treg细胞表达增高,FOXP3蛋白在肿瘤组织中表达与CD4+CD25+Treg细胞的变化相平行,可能与肿瘤多灶性有关。Objective To investigate the levels of CD4＋CD25＋regulatory T cells（Treg） and forkhead/winged helix transcription factor（FOXP3） and their clinical significance in hepatocellular carcinoma（HCC） patients.Methods Peripheral blood mononuclear cells（PBMCs） from HCC patients and healthy controls were analyzed for Treg frequency using flow cytometry.The expression of FOXP3 protein in tumor tissue was examined by immunohistochemistry and Western blotting.Results The proportion of Treg cells in peripheral blood was significantly higher in HCC group than in control group[（16.76±5.53）% vs.（6.04±2.01）%,P〈0.01].The number of FOXP3 positive cells was higher in HCC group than in paracancerous group[（103.95±34.31）/mm2 vs.（29.61±4.65）/mm2,P〈0.05].The expression of FOXP3 protein also increased in HCC.The variation in the number of Tregs correlated positively with that of more foci in tumors.Conclusion The proportion of Treg cells in peripheral blood with HCC patients increased.The levels of FOXP3 protein also increased in HCC,which paralleled the variation in the number of Tregs.The variation in the number of Tregs correlated positively with that of more foci of tumor.

关 键 词：肝细胞癌 CD4+CD25+调节性T细胞 叉状头/翅膀状螺旋转录因子 

分 类 号：R735.75[医药卫生—肿瘤]