猪用复合微生态制剂制备及保存期内活菌数研究  

The Preparation of Compound Probiotics and the Number of Living Bacterium Research in Keeping Time for Swine

作　　者：张军[1] 刘宇[1,2] 朱战波[1] 杨焕民[1] 

机构地区：[1]黑龙江八一农垦大学动物科技学院,大庆163319 [2]黑龙江省兽医科学研究所

出　　处：《黑龙江八一农垦大学学报》2011年第1期63-65,87,共4页journal of heilongjiang bayi agricultural university

基　　金：黑龙江省农垦总局科技计划课题(HNKXIV-08-03-06);黑龙江省科技厅国际科技合作项目(WB06B06)

摘　　要：为了确定两种猪肠道益生菌的固体发酵效果,以及复合微生态制剂在不同保存条件下的活菌数变化。对猪肠道益生菌干酪乳杆菌、罗伊氏乳酸杆菌进行固体发酵,测定其活菌数,然后按照一定比例将两种益生菌与液体发酵的纳豆芽孢杆菌冻干菌粉混合制成复合微生态制剂;测定该制剂在4℃和室温保存状态下14 d内及半年内益生菌活菌数量的变化。保存14 d内的微生态制剂,室温条件下活菌数先增加后逐渐减少,而4℃保存的制剂活菌数缓慢减少。室温保存状态下的微生态制剂活菌数半年内迅速减少,4℃保存的微生态制剂益生菌死亡的速度比室温保存的略微缓慢,但差异不显著(p>0.05)。半年后对4℃和室温保存状态下保存的益生菌活菌数计数结果分别为1.3×109CFU.g-11、.5×109CFU.g-1,微生态制剂活菌数仍能超过3×108CFU.g-1,可以继续使用。研究表明复合微生态制剂中的乳酸菌在4℃和室温条件下均能保存活性半年以上。In order to determine the effect of the pig intestines probiotics II solid-state fermentation,and the effect of compound probiotics preservation conditions on different living bacterium number,pig intestines probiotics Lactobacillus casei,Lactobacillus reuteri fermentation were solidified,the number of bacteria,and then live in certain proportion will be two kinds of probiotics and liquid fermentation of freeze-drying Bafillus natto sieved flour into composite probiotics,determination of the preparation room in 4 degrees and preservation condition within 14 days and half years probiotic bacteria living quantity changed.Within 14 days of probiotics,temperature conditions living bacterium number increased,and gradually decreased beyond four degrees of preparations for preservation living bacterium slowly decreased.Temperature preservation condition of probiotics living bacterium,4 ℃ and preservation of degrees of probiotics death rate than the temperature preservation slightly slower,but the difference was not significant（P 0.05）.After six months of two ways to save the probiotic bacteria living bacterium number count results respectively 109 CFU/g,1.3 ×109 CFU/g,1.5×109 CFU/g live probiotics bacterium more than 3×108 CFU/g,can were still in use.Research showed that the compound of probiotics at 4 degrees Celsius temperature and lactobacillus conditions can retain activity for more than six months.

关 键 词：猪 微生态制剂 干酪乳杆菌 罗伊氏乳酸杆菌 纳豆芽孢杆菌 保存期 

分 类 号：S482[农业科学—农药学]