复方青黛颗粒对溃疡性结肠炎模型大鼠结肠toll样受体2,4基因表达的影响  

Qingdai Granules down-regulate TLR2,TLR4 mRNA expression in the colonic mucosa of rats with ulcerative colitis

作　　者：程晓磊[1] 杜立阳[1] 刘清芳[1] 陈铭诗[1] 李鲜明[1] 仇靖[1] 

机构地区：[1]中国医科大学第一附属医院中医科,辽宁沈阳110016

出　　处：《中国中西医结合消化杂志》2011年第1期14-17,共4页Chinese Journal of Integrated Traditional and Western Medicine on Digestion

基　　金：辽宁省自然科学基金资助项目(20092130)

摘　　要：[目的]观察复方青黛颗粒对溃疡性结肠炎(UC)模型大鼠结肠toll样受体2,4(TLR2,TLR4)基因表达的影响,探讨其治疗UC的可能作用机制。[方法]用三硝基苯磺酸(TNBS)制备UC大鼠模型,将52只SD实验大鼠随机分为正常对照(空白)组、模型组、柳氮磺胺吡啶(SASP)组(500 mg/kg),复方青黛颗粒低(600 mg/kg)、中(900 mg/kg)、高(1 200 mg/kg)剂量组,从造模后第3天开始分别每天灌胃给药1次至实验结束,第14 d(灌胃10 d后),用逆转录聚合酶链反应(RT-PCR)法检测TLR2、TLR4的基因表达水平。[结果]模型组TLR2、TLR4基因相对表达量均明显高于空白组(P<0.01);复方青黛颗粒中、高剂量组TLR2相对表达量及高剂量组TLR4相对表达量与SASP组比较差异均无统计学意义,但均明显低于模型组(均P<0.05)。[结论]复方青黛颗粒能有效治疗TNBS诱导的UC模型大鼠,可能与复方青黛颗粒抑制TLR2、TLR4基因表达有关。[Objective]To investigate the effects of Qingdai Granules（QDG）on the expression of TLR2,TLR4 mRNAs in the colonic mucosa of rats with ulcerative colitis.[Methods]Ulcerative colitis was induced in rats by giving an enema of trinitrobenzene sulphonic acid（TNBS）and ethanol.Fifty two experimental animals were randomly divided into six groups：normal group,model group,sulfasalazine（SASP）group（500 mg/kg）,and low,medium and high-dose QDG groups（600,900and 1 200 mg/kg）.Except the normal group,the other groups were given intragastrically normal saline,SASP,and different concentrations of QDG,respectively,during the 3rd^12th days after model was established.Thereafter,rats were killed and the expression of TLR2 and TLR4 mRNAs in the colonic of rats was detected by reverse transcription-polymerase chain reaction（RTPCR）.[Results]Compared with the normal group,the expression of TLR2 and TLR4 mRNAs in the colon of rats in the model group was significantly up-regulated.Compared with the model group,the expression of TLR2 mRNA in the colon of rats in the medium and high-dose QDG groups was significantly down-regulated and the expression of TLR4 mRNA in the high-dose QDG group was significantly down-regulated.[Conclusion]QDG can exert protective effects against rat ulcerative colitis perhaps by down-regulating TLR2 and TLR4 mRNAs expression.

关 键 词：结肠炎 溃疡性 复方青黛颗粒 TOLL样受体2 TOLL样受体4 

分 类 号：R574.62[医药卫生—消化系统]