嗜酸乳杆菌胞壁粗提物及DNA对实验性结肠炎结肠上皮NF-κB表达的影响  

Effects of L.Acidophilus BCW and DNA on Expression of NF-κB in Murine with Acute Ulcerative Colitis

作　　者：练光辉[1,2] 陈旻湖[1] 彭侠彪[2] 阮巍山[2] 叶建明[2] 

机构地区：[1]中山大学附属一医院消化内科,广东广州510080 [2]中山市人民医院消化内科,广东中山528403

出　　处：《现代生物医学进展》2011年第3期468-471,共4页Progress in Modern Biomedicine

基　　金：中山市科技局资助项目(20091A060)

摘　　要：目的:研究嗜酸乳杆菌BCW和DNA对溃疡性结肠炎小鼠结肠粘膜上皮NF-κB表达的影响。方法:雌性BABL/c小鼠40只随机分为正常组、嗜酸乳杆菌BCW组、嗜酸乳杆菌DNA组和生理盐水对照组,除正常组小鼠外,其余各组自由饮用1.5%DSS7天建立小鼠结肠炎模型,随即给予嗜酸乳杆菌BCW(20ug/10g)、嗜酸乳杆菌DNA(0.2ug/10g)和生理盐水灌肠7天,每天观察小鼠情况,实验结束时处死小鼠,去结肠组织行HE染色观测结肠炎症情况,并行结肠上皮NF-κB免疫组化。结果:饮用DSS小鼠DAI积分显著增高,结肠组织粘膜破坏、炎症细胞浸润,结肠上皮NF-κB表达增加(9.15±0.43),嗜酸乳杆菌BCW和DNA能降低小鼠DAI积分,减轻粘膜损伤,降低结肠上皮NF-κB的表达(4.67±0.56/6.03±0.60)。结论:嗜酸乳杆菌BCW和DNA能缓解急性溃疡性结肠炎,降低结肠上皮NF-κB的表达。Objective：To investigate the effect of L.acidophilus BCW and DNA on the expression of NF-κB in murine intestinal mucosa with UC.Methods：40 specific pathogen free,BALB/c female mice were randomly divided into acute normal group,L.aci-dophilus BCW-treated group,L.acidophilu DNA-treated group and NS control group.Mice were induced by 1.5% DSS water for 7 days free drinking except nomal group.The mice were respectively received L.acidophilus BCW（20ug/10g）,L.acidophilu DNA（0.2ug/10g） and physiological saline,while mice of normal control group were untreated.The animal were recorded daily and killed after 7days for assaying the NF-κB with immunohistochemical staining.Results：Both DAI scores and leisions of colonic mucosa of DSS-induced mice decreased markedly by L.acidophilus BCW and it＇s DNA,while they were more severe than that of normal.Compared with the model control group（9.15±0.43）,the expression of NF-κB decreased significantly（BCW：4.67±0.56,DNA：6.03±0.60）,but which was still higher than that of normal.Conclusion：The L.acidophilus BCW and it＇s DNA alleviate DSS-induced ulcerative colitis and decreased the expression of NF-κB in intestinal mucosa.

关 键 词：溃疡性结肠炎 乳酸杆菌 胞壁成分 DNA 细胞核因子-ΚB 

分 类 号：R574.62[医药卫生—消化系统]