食管癌Eca109细胞中Nucleostemin基因表达的实验研究  

THE EXPERIMENTAL STUDY OF NUCLEOSTEMIN GENE EXPRESSION IN ESOPHAGEAL CANCER ECA109 CELLS

作　　者：郑学芝[1,2] 金秀东[1,2] 张绪东[1,2] 刘桂莲[1,2] 孙卫[1,2] 念红[1,2] 李丽[1,2] 徐秋玲[1,2] 蔡子微 

机构地区：[1]牡丹江医学院生理教研室,黑龙江牡丹江157011 [2]黑龙江省高校肿瘤疾病防治重点实验室,黑龙江牡丹江157011

出　　处：《牡丹江医学院学报》2011年第1期3-5,共3页Journal of Mudanjiang Medical University

基　　金：黑龙江省普通高等学校青年学术骨干支持计划项目(No.1153G047)

摘　　要：目的:研究Nucleostemin(NS)基因在食管癌Eca109细胞中的表达及凋亡作用。方法:提取各组食管癌细胞总RNA,用半定量RT-PCR方法检测NS基因的表达情况;用CCK-8法检测3组细胞增殖抑制率;TUNEL试剂盒法检测细胞凋亡情况。结果:与对照组比较,实验组细胞NS基因表达量下降,细胞增殖抑制率>65%,细胞凋亡增加(凋亡指数为39.47%)差别具有统计学意义。结论:NS基因特异性RNA干扰使食管癌Eca109细胞中NS基因表达量下降,出现细胞增殖抑制,细胞凋亡增加。Objective：To detect the NS gene expression and cell apoptosis in esophageal cancer Eca109 cells.Methods： Total RNA was isolated from esophageal cancer Eca109 cells,measured the NS gene expression by RT-PCR;then detected the cell proliferation by CCK-8 assay;detected the cell apoptosis by TUNEL kit.Results：compared with the two control groups,the NS gene expression in silencer group cells reduced,the proliferation inhibitory rate was higher than 65%,cell apoptosis increased.Conclusion：the NS gene specific RNAi can reduce the level of NS gene expression and inhibit esophageal cancer Eca109 cells proliferation and increase cell apoptosis.

关 键 词：NUCLEOSTEMIN 食管癌 RNA干扰 凋亡 

分 类 号：R737.9[医药卫生—肿瘤]