乳腺癌组织中14-3-3σ、P53和IKKα基因mRNA表达及其意义  

The mRNA expression of 14-3-3σ、P53 and IKKα gene in breast cancer tissue and its significance

作　　者：范昭[1] 张信[1] 陈启明[1] 叶静[2] 

机构地区：[1]北京大学深圳医院急诊科,深圳518036 [2]北京大学深圳医院中心实验室

出　　处：《陕西医学杂志》2011年第3期267-269,共3页Shaanxi Medical Journal

基　　金：广东省深圳市科技计划项目(No.200803003)

摘　　要：目的:通过研究14-3-3σ、P53和IKKα基因在散发性乳腺癌中mRNA表达情况,探讨这三个基因在乳腺癌发生中的作用。方法:采用SYBR greenⅠ实时定量PCR法检测14-3-3σ、P53和IKKα基因mRNA表达水平。结果:采用相对量分析法,14-3-3σ、P53和IKKα基因的△△CT依次为0.77、1.96和0.02,根据样本平均相对含量%=2-△△CT,得出14-3-3σ、P53和IKKα基因在癌症组平均转录表达量依次是非癌组的58%、25%和98%,经统计学分析,差异没有显著性。结论:14-3-3σ和P53基因在乳腺癌组织中是低表达的,IKKα基因mRNA表达水平不受影响,三个基因在乳腺癌的发生中是相互影响的。Objective：To study the mRNA expression of 14-3-3σ、P53 and IKKα gene and to investigate the role of these genes in sporadic breast cancer.Methods：The mRNA expression of 14-3-3σ、P53 and IKKα gene were detected by SYBR green Ⅰreal time PCR.Results：△△CT of 14-3-3σ、P53 and IKKαgene was 0.77、1.96 and 0.02 in turn by the relative amount analysis.According to the sample average relative amount%=2-△△CT,the average expression level in cancer was 58% of 14-3-3σ、25% of P53 and 98% of IKKαgene in non-cancer.The difference was not significant.Conclusion：The expression level of 14-3-3σ and P53 gene were low in breast cancer.The expression of IKKα gene is not affected.The three genes influence each other in breast cancer.

关 键 词：乳腺肿瘤/免疫学 @14-3-3σ基因 基因 P53 @IKKα基因 mRNA 信使基因表达 

分 类 号：R737.9[医药卫生—肿瘤]