用电喷雾质谱法监测重组人肿瘤坏死因子中二硫键的还原过程  

ESI-MS Dynamic Monitoring of the Reduction Process of Disulfide Bond in rhTNF

作　　者：董方霆[1] 邱丰和[1] 廖杰[1] 桑志红[1] 杨松成[1] 

机构地区：[1]国家生物医学分析中心

出　　处：《分析测试学报》1999年第3期56-58,共3页Journal of Instrumental Analysis

摘　　要：应用电喷雾质谱方法动态监测了用巯基乙醇及二硫苏糖醇还原重组人肿瘤坏死因子中二硫键的全过程，表明使用巯基乙醇还原二硫键速度较二硫苏糖醇快，并且在还原过程中有质量加和产物生成，还原过程易于观察，可为进一步对蛋白质酶切进行结构鉴定提供监测方法；利用该法还可测定分子中二硫键的数目。A ESI-MS method was used to dynamically monitor the process of reduction of internal disulfide bond in rhTNF by 2 _ mercaptoethanol(2 _ ME) and DTT respectively. The results indicate that the reduction with 2 _ ME is monitored much faster, and easier due to the mass increase in intermediate product compared with DTT. It can provide a method for further research of enzyme digestion to identify the primary structure of the protein. The number of the disulfide bond in molecular can be also determined by this method.

关 键 词：二硫键 还原 重组 人肿瘤坏死因子 RHTNF ESIMS 

分 类 号：Q510.3[生物学—生物化学]