STR基因多态性与巴马地区老人长寿的关系  

Research on correlation of STR polymorphisms and longevity in Bama county

作　　者：张志勇[1] 容敏华[1] 臧宁[2] 廖明[2] 胡天桥[1] 黎湘娟[1] 覃健[1] 农清清[1] 张楠[1] 肖德强[1] 韦世录[2] 李刚[2] 吕泽平 黄和明[4] 何敏[2] 

机构地区：[1]广西医科大学公共卫生学院,广西南宁530021 [2]广西医科大学医学科学实验中心 [3]广西江滨医院 [4]广西山区综合技术开发中心

出　　处：《中国老年学杂志》2011年第4期551-554,共4页Chinese Journal of Gerontology

基　　金：国家自然科学基金资助项目(30560166);广西科学研究与技术开发项目(桂科能0815011-6-5);广西科学基金资助项目(桂科基0639045;桂科自0991115);广西自然科学基金(桂科青0832031)

摘　　要：目的探讨广西巴马长寿老人TH01等15个STR多态位点与长寿的关系。方法调查巴马长寿区长寿老人167例(长寿组),非长寿区的长寿老人22例(对照1组),非长寿区无长寿家族史的健康对照者134例(对照2组)。采用多色荧光标记的聚合酶链反应-短串联重复序列(PCR-STR)技术及自动测序法,检测各组人群TH01等15个STR位点等位基因分型。比较长寿组和各对照组等位基因及基因型频率的差异。结果长寿组分别与各对照组相比频率有差异(P<0.05)的等位基因或基因型如下:TH01-A10、CSF1PO-A9、TH01A9-10频率低于对照1组;D19S433-A17、D7S820-A10、D7S820-A11频率高于对照2组,D7S820-A12/A13/A14三个等位基因、vWA-A17、D8S1179-A15、D5S818 A10-12低于对照2组。结论巴马长寿群体有着自己特有的、异于其他地区长寿群体的STR等位基因分布。在巴马长寿人群中发现了TH01、CSF1PO、vWA、D19S433、D7S820、D5S818、D8S1179等位点等位基因的置换或突变可能与长寿或衰老的进程相关。Objective To explore the correlation of 15 short tandem repeats （STR） loci containing TH01 and natural longevity in Bamaa county. Methods 167 longevous individuals from longevity region in Bama were recruited as the longevity group. In Bama non-longevity region, 22 longevous individuals were selected as control group 1 and control group 2 was composed of 134 residents without longevity family history. Muhiplex PCR amplification and genetic analyzer were applied to detect and analyze the genetic polymorphisms of 15 STR loci. Chi square test was performed to analyze the differences of frequencies of alleles and genotypes between longevity group and each control group. Results There were statistically significant changes of some frequencies of alleles and genotypes between longevity group and each control group （P 〈0. 05）. Decreased frequencies of TH01-A10, CSF1PO-A9, TH01 A9-10 were observed in longevity group, when compared with that in control group 1. In the longevity group, the frequencies of D19S433-A17, D7S820-A10, D7S820-A1 1 were higher and fre- quencies of D7S820-A12/A13/A14, vWA-A17, D8S1179-A15, D5S818 A10-12 were lower than those in control group 2. Conclusions The unique STR allele distributions in Bama longevous population differ from that of longevous population in other geographic areas. The replacement or mutation of TH01, CSF1PO, vWA, D19S433, DTS820, D5S818, D8S1179 loci may be related to longevity and aging-related processes.

关 键 词：长寿 短串联重复序列 基因多态性 

分 类 号：R394[医药卫生—医学遗传学]