丙泊酚对内毒素性大鼠急性肺损伤的保护作用  

The protective effect of propofol on lipopolysaccharide-induced acute lung injury in rats

作　　者：李莎[1] 张焰[1] 彭生[1] 徐鹏程[2] 

机构地区：[1]苏州大学附属第四人民医院麻醉科,无锡市214062 [2]徐州医学院麻醉实验室江苏省麻醉学重点实验室

出　　处：《江苏医药》2011年第3期263-265,共3页Jiangsu Medical Journal

基　　金：江苏省无锡市卫生局科研项目(XM0805)

摘　　要：目的探讨丙泊酚对内毒素(LPS)性大鼠急性肺损伤(ALI)的影响。方法 60只SD大鼠随机均分5组:A组为ALI模型;B1、B2、B3组静脉注射LPS5mg/kg后,再分别输注丙泊酚5、10、15mg.kg-1.h-1;C组为假手术对照。放血处死大鼠,右肺HE染色,左肺测定湿干重比;分离外周血中性粒细胞(PMN),流式细胞仪检测PMN凋亡。结果与C组比较,A组肺部损伤严重,肺泡损伤比值(IQA)显著增加、PMN的凋亡率显著降低(P<0.05);与A组比较,丙泊酚处理组肺损伤程度减轻,IQA降低,凋亡显著增加,且呈剂量依赖性(P<0.05)。结论丙泊酚减轻LPS诱导的大鼠ALI,这可能与促进PMN的凋亡有关。Objective To investigate the effect of propofol on lipopolysaccharide （LPS）-induced acute lung injury （ALI） in rat.Methods Sixty rats were randomly divided into five groups with 12 rats each.Group A was ALI model.Groups of B1,B2 and B3 were intravenously injected LPS 5mg/kg,which was followed by propofol infusion in a dose of 5,10 or 15mg kg-1·h-1,respectively.Group C was sham operated.After killed by exsanguinations,right lung of rat was stained by HE and left lung was taken for measurement of the ratio of wet to dry weight（W/D）.PMNs were isolated from peripheral blood and apoptosis was determined by flow cytometry.Results Compared with group C,lung injury was more serious in group A with increased IQA and decreaed apoptosis of PMN（P0.05）.Compared with group A,the lung injury in propofol intervention groups was slighter with decreased IQA and increased apoptosis of PMN in a dose-dependent manner（P0.05）.ConclusionPropofol can attenuate ALI induced by LPS in rats,which may be related to promoting apoptosis of PMN.

关 键 词：丙泊酚 急性肺损伤 中性粒细胞 凋亡 

分 类 号：R363[医药卫生—病理学]