骨髓间充质干细胞对免疫介导再生障碍性贫血的治疗  

Bone marrow mesenchymal stem cells for the treatment of immune induced aplastic anemia

作　　者：黄颖[1] 郑翠玲[2] 杨少光 韩忠朝 

机构地区：[1]昆明医学院第一附属医院血液科,云南省昆明市650031 [2]中国医学科学院肿瘤医院检验科,北京市100021 [3]中国医学科学院天津血液学研究所重点实验室,天津市300020

出　　处：《中国组织工程研究与临床康复》2011年第1期41-45,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：云南省科技计划项目(2008ZC134M);课题名称:脐带间充质干细胞对再生障碍性贫血患者T细胞的免疫调节作用机制研究~~

摘　　要：背景:骨髓间充质干细胞具有免疫调节作用,可以影响淋巴细胞和其他免疫细胞的功能,同时其分泌的因子尚有支持造血的作用。而再生障碍性贫血的发生、发展与T细胞功能亢进引起的造血干、祖细胞凋亡密切相关。目的:比较骨髓间充质干细胞和全骨髓细胞治疗免疫介导再生障碍性贫血小鼠的疗效。方法:建立BALB/C小鼠免疫介导再生障碍性贫血模型。将BALB/C小鼠随机分成正常组、对照组、注射骨髓间充质干细胞和注射全骨髓细胞组。观察骨髓间充质干细胞和全骨髓细胞对实验动物的疗效及骨髓的造血组织容量、脂肪细胞和血窦结构。结果与结论:再生障碍性贫血模型小鼠至第20天,对照组全部死亡,骨髓间充质干细胞组4例死亡,全骨髓细胞组1例死亡。②骨髓间充质干细胞组和全骨髓细胞组存活小鼠,其体质量变化趋势、血细胞计数、骨髓造血功能恢复均无显著性差异。③检测骨髓间充质干细胞组和全骨髓细胞组脾脏CD4+T细胞、CD8+T细胞、CD4+/CD8+的比值示无差异,与正常小鼠相比,骨髓间充质干细胞组外周血CD4+T细胞、CD8+T细胞水平有下降趋势。全骨髓细胞组CD8+T细胞水平明显下降。提示,单独使用骨髓间充质干细胞治疗能够使部分再生障碍性贫血模型小鼠症状改善、造血恢复。骨髓间充质干细胞治疗有效率不如全骨髓细胞,但两组无显著性差异。BACKGROUND：Bone marrow mesenchymal stem cells （BMSCs） have immunomodulatory effects on lymphocytes and other immune-associated cells. Supportive effects of BMSCs on hematopoietic cells had been certified. Occurrence and development of aplastic anemia are closely associated with T cell hyperfunction-induced hematopoietic stem/progenitor cells. OBJECTIVE：To compare the therapeutic effects of BMSCs and whole bone marrow cells in immune-induced aplastic anemia mice. METHODS：Immune-induced aplastic anemia BALB/C mouse models were established. The BALB/C mice were randomly assigned to normal,control,BMSC injection and whole bone marrow cell groups. Therapeutic effects of BMSCs and whole bone marrow cells,volume of hemopoietic tissues,adipocytes and blood sinus structure were observed. RESULTS AND CONCLUSION：①On day 20,all mice without treatment （8/8） and 4 of 8 mice which received BMSCs died,while 7 of 8 mice were infused with bone marrow cells and kept live. ②No significant difference in change of weight,blood cells,haematopoiesis was detected between BMSCs or bone marrow cells. ③In spleen cells of improved mice with BMSCs or bone marrow cells,the percentages of CD4＋T cells,CD8＋T cells and the ratio of CD4＋/CD8＋ were similar. In peripheral blood,the percentages of CD4＋T cells and CD8＋T cells were lower in BMSCs group than in normal group. The level of CD8＋T cells was lowest in mice with bone marrow cells. These results indicate that BMSCs improve aplastic anemia symptom and recover hematopoiesis. The bone marrow cells were more effective than BMSCs in the therapeutical effect,but no significant difference was determined.

关 键 词：再生障碍性贫血 骨髓间充质干细胞 BALB/C鼠 移植 免疫 

分 类 号：R394.2[医药卫生—医学遗传学]