自体骨髓间充质源神经干细胞移植应用于中枢神经系统疾病7例  

Autologous transplantation of bone marrow mesenchymal cells-derived neural stem cells for treating seven patients with central nervous system diseases

作　　者：曹志勇[1] 刘涵[1] 单君君[1] 朱孟慧[1] 王俊明[1] 陈昊[1] 程言博[1] 樊红彬[1] 董瑞国[1] 耿德勤[1] 

机构地区：[1]徐州医学院附属医院神经内科,江苏省徐州市221002

出　　处：《中国组织工程研究与临床康复》2011年第1期163-166,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:前期应用骨髓间充质源神经干细胞移植治疗模拟神经系统疾病动物实验模型取得较好效果。目的:观察应用自体骨髓间充质源神经干细胞移植治疗中枢神经系统疾病的临床疗效。方法:2008-10/2009-04应用自体骨髓间充质源神经干细胞移植治疗中枢神经系统疾病患者7例。骨髓穿刺术抽取自体骨髓血50～60mL,在体外经过分离培养后,制成骨髓间充质干细胞悬液,由患者自体脑脊液诱导定向分化为神经干细胞,通过腰椎穿刺术经蛛网膜下腔鞘内注射输注方式移植。观察患者移植前后神经功能缺损症状及移植后不良反应。结果与结论:经移植治疗后3例患者均未见不良反应。出院后随访6个月,7例患者神经功能缺损症状较移植前均有一定程度的改善。提示自体骨髓间充质源神经干细胞移植治疗中枢神经系统疾病所致的神经功能缺损疾病是一种安全、方便、有效的方法。BACKGROUND：Previous studies of animal models of nervous system diseases have shown that bone marrow mesenchymal cells-derived neural stem cells have a good effect on central nervous system diseases. OBJECTIVE：To observe the therapeutic effects of autologous transplantation of bone marrow mesenchymal cells-derived neural stem cells applied for central nervous system diseases. METHODS：Seven patients with central nervous system diseases were subjected to by autologous transplantation of bone marrow mesenehymal cells-derived neural stem cells from October 2008 to April 2009. Bone marrow puncture aspiration was used to collected 50-60 mL bone marrow blood,which was prepared into bone marrow mesenchymal stem cell suspension following isolation and culture in vitro. Neural stem cells were isolated and induced with self cerebrospinal fluid,and transplanted into patients by lumbar puncture via the subarachnoid cavity. Neurological impairment and side effects posttransplantation were observed prior to and following transplantation. RESULTS AND CONCLUSION：No adverse reactions were found in three patients after transplantation. The neurological function of the seven patients had been improved in 6-month follow-up. Results suggest that it is a safe,convenient and effective method to use autologous transplantation of bone marrow mesenchymal cells-derived neural stem cells to treat patients with neurological impairment induced by nervous system diseases.

关 键 词：神经干细胞 骨髓 移植 自体 中枢神经系统疾病 

分 类 号：R394.2[医药卫生—医学遗传学]