CpG寡脱氧核苷酸对外周血单个核细胞功能的影响：1型糖尿病患者与健康者对照  

Effects of CpG oligonucleotides on the function of peripheral blood mononuclear cells in patients with type 1 diabetes mellitus versus healthy volunteers

作　　者：田德增[1] 刘明哲[1] 张永选[1] 魏晓华[1] 任保仙[1] 王海敏[1] 

机构地区：[1]河南省濮阳市安阳地区医院内分泌科,河南省安阳市455000

出　　处：《中国组织工程研究与临床康复》2011年第1期183-186,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:有研究表明CpG寡脱氧核苷酸可增强外周血单个核细胞的功能,但对1型糖尿病患者的影响至今少有报道。目的:观察CpG寡脱氧核苷酸对1型糖尿患者患者与健康志愿者外周血单个核细胞γ干扰素、白细胞介素12,10表达的影响。方法:将1型糖尿病患者与健康志愿者的外周血单个核细胞根据刺激物不同分为空白对照组、CpG寡脱氧核苷酸组。用RT-PCR法检测外周血单个核细胞γ干扰素、白细胞介素12mRNA和白细胞介素10mRNA的表达。结果与结论:1型糖尿患者γ干扰素和白细胞介素12mRNA的表达明显低于健康志愿者(P<0.01)。1型糖尿患者与健康志愿者外周血单个核细胞经CpG寡脱氧核苷酸组刺激后,γ干扰素和白细胞介素12mRNA的表达增高(P<0.01),白细胞介素10mRNA的表达无差异(P>0.05)。结果提示,CpG寡脱氧核苷酸可促进1型糖尿患者外周血单个核细胞表达γ干扰素和白细胞介素12。BACKGROUND：CpG oligonucleotide has been shown to strengthen the function of peripheral blood mononuclear cells （PBMCs）,but its effects on type 1 diabetes mellitus has been rarely reported. OBJECTIVE：To investigate the effects of CpG oligonucleotide on the expression of interferon γ （IFN-γ）,interleukin （IL） -12 and IL-10 in PBMCs in patients with type 1 diabetes mellitus versus healthy controls. METHODS：PBMCs were isolated from patients with type 1 diabetes mellitus and healthy controls and then cultured in RPMI-1640 with non-stimulator （control group） and CpG oligonucleotide （CpG oligonucleotide group）,respectively. The mRNA expression of IFN-γ,IL-10,and IL-12 in PBMCs was detected by reverse transcription-polymerase chain reaction. RESULTS AND CONCLUSION：mRNA expression of IFN-γ and IL-10 was significantly lower in patients with type 1 diametes mellitus than in healthy controls （P 0.01）. In the CpG oligonucleotide group,the mRNA expression of IFN-γand IL-12 was significantly higher than in the healthy control group （P 0.01）,but the mRNA expression of IL-10 was similar to that in the healthy control group （P 0.05）. These findings demonstrated that CpG oligonucleotide can promote the production of IFN-γ and IL-12 in PBMCs of type 1 diametes mellitus.

关 键 词：CPG寡脱氧核苷酸 Γ干扰素 白细胞介素 1型糖尿病 

分 类 号：R394.2[医药卫生—医学遗传学]