新型纳米银/聚氨酯胆道支架表面抗菌涂层的体外抑菌试验  

In vitro antibacterial test of the surface antibacterial coating of a new nano-silver/polyurethane biliary stent

作　　者：何伟[1] 王翔[1] 高莹[1] 梁军才[1] 于亭亭[1] 范志宁[1] 

机构地区：[1]南京医科大学第二附属医院消化医学中心,江苏省南京市210011

出　　处：《中国组织工程研究与临床康复》2011年第3期453-456,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：国家高技术研究发展计划(863计划)(2007AA02190)资助;课题名称:银系抗菌亲水胆管内支架的研制~~

摘　　要：背景:胆管支架已广泛应用于胆管各种良恶性狭窄的姑息治疗,临床短期疗效显著,但长期疗效由于支架再狭窄而受到限制。目的:研制新型纳米银/聚氨酯胆管内支架复合抗菌涂层并检测其体外抗菌性能。方法:原位还原法用纳米银、聚氨酯制备纳米银/聚氨酯抗菌材料,采用抑菌环试验法分别测定实验组(纳米银/聚氨酯)、阳性对照(硝酸银纸片)和阴性对照(普通聚氨酯材料)3组对金黄色葡萄球菌、大肠埃希菌、铜绿假单胞菌、肺炎克雷伯杆菌和肠球菌的抑菌环直径并记录分析数据。结果与结论:纳米银/聚氨酯在体外对金黄色葡萄球菌、大肠埃希菌、铜绿假单胞菌、肺炎克雷伯杆菌和肠球菌均有抑制效果,与硝酸银纸片相比,抑菌效果差异无显著性意义。结果显示纳米银/聚氨酯复合物具有良好的抗菌效果,且具有较好的稳定性。BACKGROUND： Biliary stents have been widely used in a variety of benign and malignant bile duct stricture as a palliative treatment. Its short-term efficacy is significant, but long-term use is limited by stent restenosis. OBJECTIVE： To produce a new antibacterial coating used in biliary stent by means of nano-silver/polyurethane, and to test in vitro antibacterial properties. METHODS： Firstly, composite materials were made of nano-silver and polyurethane by in situ reduction method. Antibacterial test was used to detect in vitro antibacterial properties against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae and Enterococci of three groups： experimental group （nano-silver/polyurethane）, positive control group （filter paper soaked in silver nitrate solution） and negative control group （polyurethane）, antibacterial zone diameters were measured and analyzed. RESULTS AND CONCLUSION： The difference was not significant between experimental group and positive control group in the antibacterial effect on Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae and Enterococci. Nano-silver/polyurethane composite has good antibacterial property and better stability.

关 键 词：纳米银 聚氨酯 复合材料 抑菌 应用 

分 类 号：R318[医药卫生—生物医学工程]