EB病毒潜伏膜蛋白1和鼻咽癌上皮间质转化相关分子与颈淋巴结转移关系  

Latent membrane protein-1 of EB virus and the phenotype of epithelial-mesenchymal transition and cervical lymph node metastasis in nasopharyngeal carcinoma

作　　者：岳利华[1] 蒋志毅[1] 邬万新[2] 张燕萍[2] 殷鹏[1] 张亚军[1] 盛成[1] 魏国彪[1] 李湘[1] 凌恺[1] 

机构地区：[1]浙江嘉兴学院附属第一医院耳鼻咽喉科,浙江嘉兴314000 [2]浙江嘉兴学院附属第一医院病理科

出　　处：《临床耳鼻咽喉头颈外科杂志》2011年第6期270-273,共4页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

基　　金：浙江省嘉兴市科技局资助项目(No:2008AY2033-11)

摘　　要：目的:评价EB病毒潜伏膜蛋白1(LMP-1)和鼻咽癌上皮间质转化相关分子与颈淋巴结转移关系。方法:88例鼻咽癌活检标本依据病理检查或MRI检查分成病理转移组(18例)、MRI转移组(40例)和无转移组(30例)。免疫组织化学检测鼻咽癌组织中潜伏膜蛋白1(LMP-1)、STAT3、Twist、E-cadherin和Vimentin的表达情况。结果:88例鼻咽癌组织中,LMP-1阳性表达见于35例,阳性率为38.7%。病理转移组、MRI转移组和无转移组的LMP-1阳性率分别为38.9%(7/18)、47.5%(19/40)和30.0%(9/30),3组间差异无统计学意义。LMP-1表达分别与Twist和Vimentin表达呈正相关(r=0.276和0.282,均P<0.01),与STAT3和E-Cadherin表达无相关性。88例鼻咽癌组织中STAT3、Twist和Vimentin阳性率以及E-Cadherin异常表达率分别64.8%(57/88)、54.5%(48/88)、20.0%(22/88)和60.2%(53/88)。STAT3、Twist和Vimentin阳性率以及E-Cadherin异常表达率在病理转移组、MRI转移组和无转移组间差异均分别有统计学意义(均P<0.05)。STAT3表达分别与Twist和Vimentin表达均呈正相关性(r=0.712和0.316,均P<0.01)。结论:EMT在鼻咽癌转移过程中起着重要作用。LMP-1可能仅作为上游影响因素之一间接影响EMT的发生,但与颈部淋巴结转移可能无直接相关性。Objective：To evaluate the relation of EB virus latent membrane protein-1（LMP-1） and the phenotype of epithelial-mesenchymal transition（EMT）and cervical lymph node metastasis in nasopharyngeal carcinoma.Method：Based on histopathology and MRI imaging,nasopharyngeal biopsy tissues from 88 patients with nasopharyngeal carcinoma were divided into 3 groups： pathologic metastasis（18）,MRI metastasis（40） and without metastasis（30）.The expressions of LMP-1,STAT3,Twist,E-Cadherin and Vimentin were examined immunohistochemically in biopsy tissues.Result：LMP-1 expression was found in 35 of 88 biopsy tissues with a positive rate of 38.7%.The positive rates of LMP-1 in groups of pathologic metastasis,MRI metastasis and without metastasis were 38.9%（7/18）,47.5%（19/40） and 30.0%（9/30）,respectively,and significant difference were not found among three groups.The expression of LMP-1 was positively correlated to both expressions of Twist and Vimentin（r=0.276 and 0.282,are P0.01）,but not to both expressions of STAT3 and E-Cadherin.The positive expressions or abnormal expression of STAT3,Twist,Vimentin and E-Cadherin were found in 57 of 88（64.8%）,48 of 88（54.5%）,22 of 88（20.0%）and 53 of 88（60.2%）,respectively.Significant differences in the expression of STAT3,Twist,Vimentin and E-Cadherin were all found among groups of pathologic metastasis,MRI metastasis and without metastasis,respectively（are P0.05）.The expression of STAT3 was positively correlated to both expressions of Twist and Vimentin（r=0.712 and 0.316,均P0.01）.Conclusion：EMT plays important role in cervical lymph node metastasis in nasopharyngeal carcinoma.LMP-1 may be only as one of upstream factors associated with the EMT,but not the decisive factor for cervical lymph node metastasis.

关 键 词：鼻咽肿瘤 EB病毒 潜伏膜蛋白1 上皮间质转化 转移 

分 类 号：R739.6[医药卫生—肿瘤]