HIF-1α、MMP-2在卵巢癌中的表达及其与血管生成拟态的关系  

Expression of HIF-1α and MMP-2 in vasculogenic mimicry of ovarian cancer

作　　者：苏敏[1] 张玉泉[1] 黄华[2] 施公胜[2] 孙峰[3] 邱亚萍[1] 

机构地区：[1]南通大学附属医院妇产科,南通226001 [2]南通大学附属医院病理科,南通226001 [3]南通大学附属医院血液实验室,南通226001

出　　处：《现代妇产科进展》2011年第2期83-87,共5页Progress in Obstetrics and Gynecology

基　　金：南通市社会发展科技计划资助课题(No:S2008059);国家自然科学基金青年基金资助课题(No:30801226)

摘　　要：目的：探讨卵巢癌缺氧诱导因子HIF-1α、基质金属蛋白酶MMP-2的表达及其与血管生成拟态（VM）的关系。方法：收集本院2000年1月~2009年5月经手术切除的卵巢肿瘤组织石蜡标本81例,免疫组化检测HIF-1α、MMP-2蛋白表达,采用CD34和PAS双重染色的方法检测卵巢癌组织中存在的VM,讨论HIF-1α、MMP-2表达与VM的关系。结果：卵巢癌HIF-1α表达与临床分期（r=0.392,P=0.020）、分级（r=0.358,P=0.035）呈正相关,MMP-2表达与分级（r=0.409,P=0.015）呈正相关。卵巢癌不同分级组的VM形成率有显著差异（P=0.018）,低分化的卵巢癌VM阳性率高于高分化组。VM阳性组和阴性组的HIF-1α表达有统计学差异（P=0.003）,VM阳性组和阴性组的MMP-2表达有统计学差异（P=0.004）。结论：低分化卵巢癌组织更易形成VM,HIF-1α、MMP-2表达与卵巢癌分级及VM密切相关。Objective：To investigate the relationship between expression of hypoxia induced factor-1α（HIF-1α）,matrix metalloproteinase-2（MMP-2） and vasculogenic mimicry（VM）.Methods：81 paraffin-embedded sections of ovarian tumor were studied.Expression of HIF-1α and MMP-2 were detected by immunohistochemical staining.The presence of VM was observed by histochemical double staining of CD34 and PAS.Relationship between expression of HIF-1α,MMP-2 and VM were discussed.Results：Positive expressions of HIF-1α and MMP-2 protein were detected in ovarian cancer.Vasculogenic mimicry,when PAS was positive but CD34 was negative,appeared in some cases of ovarian cancer.Positive correlation was observed between tumor grade and VM（P=0.018）.Positive relationship was found between expression of HIF-1α and clinical stage（r=0.392,P=0.020）,grade（r=0.358,P=0.035）.Positive relationship was also found between expression of MMP-2 and grade（r=0.409,P=0.015）.Expression of HIF-1α（P=0.003） and MMP-2（P=0.004） in ovarian cancer with VM were increased significantly than those without VM.Conclusion：Vasculogenic mimicry exists in ovarian cancer,especially in the lower grade.Expression of HIF-1α and MMP-2 are associated with tumor grade and formation of vasculogenic mimicry.

关 键 词：缺氧诱导因子1Α 基质金属蛋白酶2 卵巢肿瘤 血管生成拟态 

分 类 号：R737.31[医药卫生—肿瘤]