Cyclin G1在下咽鳞癌中的表达及其临床意义  

The clinical significance of Cyclin G1 in hypopharyngeal carcinoma

作　　者：陈万军[1,2] 

机构地区：[1]山东省肿瘤医院头颈科 [2]山东省医学科学院,山东济南250117

出　　处：《中国癌症杂志》2011年第2期120-124,共5页China Oncology

基　　金：山东省优秀中青年科学家科研奖励基金项目(No:2008BS03034)

摘　　要：背景与目的:细胞周期蛋白(Cyclin G1)可能参与多种恶性肿瘤的侵袭和转移,但鲜见Cyclin G1与下咽鳞癌关系的报道。本研究旨在探讨Cyclin G1在下咽鳞癌中的表达及其与临床生物学行为之间的关系。方法:取下咽鳞状细胞癌手术标本56例,正常黏膜标本14例,应用免疫组化方法检测Cyclin G1在下咽癌组织和正常黏膜中的表达;应用实时荧光定量RT-PCR检测Cyclin G1 mRNA表达。结果:免疫组化结果显示:Cyclin G1在下咽癌中表达主要位于细胞核,阳性率为53.6%(30/56),而正常黏膜未见表达(P<0.01);随着肿瘤的增大,其阳性表达率逐渐增高(P>0.05);Cyclin G1在颈部淋巴结转移组阳性率为64.9%(24/37),明显高于未转移组31.6%(6/19)(P<0.05);Cyclin G1阳性表达患者3年内复发率为60.0%,阴性表达患者3年内复发率30.8%(P<0.05);Cyclin G1阳性表达患者5年生存率为30.0%,明显低于阴性表达患者5年生存率57.7%(P<0.05)。RT-PCR检测Cyclin G1 mRNA在下咽癌组织中的相对表达量(1.081±0.302)高于正常黏膜组相对表达量(0.713±0.158)(P<0.05);中晚期原发肿瘤、颈淋巴结转移及远处转移患者Cyclin G1 mRNA表达量均略高于早期肿瘤、无颈淋巴结及远处转移患者(P>0.05),Cyclin G1 mRNA表达量越高肿瘤越容易复发(P<0.05),患者生存时间也越短(P<0.01),与免疫组化结果一致。结论:Cyclin G1在下咽鳞癌中存在高表达,其异常表达在下咽鳞癌的发生、发展中发挥着重要作用,可能是下咽鳞癌预后不良的指标之一。Background and purpose：Cyclin G1 may be involved in the progression of invasion and metastasis of many malignant tumors,but there have been few reports about the relationship between Cyclin G1 and hypopharyngeal carcinoma.This study aimed to determine the relationship between Cyclin G1 and the biological behavior of hypopharyngeal squamous cell carcinoma.Methods：Immunohistochemistry and real time quantitative polymerase chain reaction methods were applied to determine Cyclin G1 expression and gene amplification in 56 cases of hypopharyngeal squamous cell carcinoma and 14 cases of normal adjacent epithelium.The results were compared with clinical variables and patient outcomes.Results：Positive expressions of Cyclin G1 in the tumorous cell were observed in 30 cases（53.6%）,whereas no expression of Cyclin G1 was found in normal epithelium（P〈0.01）.Cyclin G1 overexpression increased gradually from T1 to T4,but no significant difference was found（P〉0.05）.Cyclin G1 immunoreactivity in cases with lymph node metastases was significantly higher than those without lymph node metastases（P〈0.05）.The recurrence rates in the patients with positive expression of Cyclin G1 were 60.0%,significantly higher than the patients with negative expression（P〈0.05）.On the other hand,the 5-year overall survival rates for the patients with positive expression were 30.0%,significantly lower than that with negative expression（P〈0.05）.The average Cyclin G1 mRNA level in patients with hypopharyngeal carcinoma were much higher than those in normal epithelium（P〈0.05）.No significant difference of Cyclin G1 mRNA expression in primary tumor,lymph node metastasis and distant metastasis were found in hypopharyngeal carcinoma（P〉0.05）.Cyclin G1 mRNA expression were significantly related with recurrence and survival time（P〈0.05）.Conclusion：Cyclin G1 is highly expressed in hypopharyngeal squamous cell carcinoma.It may play a role in the progression of hypopharyngeal squamous cell carcinoma and can p

关 键 词：CyclinG1 下咽 鳞状细胞 癌 

分 类 号：R739.8[医药卫生—肿瘤] R730.23[医药卫生—临床医学]