Hedgehog信号通路对人胃癌SGC-7901细胞侵袭迁移作用及机制探讨  

Investigation into the effect and mechanism of Hedgehog signaling pathway on invasion and migration of human gastric cancer cell line SGC-7901

作　　者：欧阳小波[1] 郝亚琴[1] 王立[1] 

机构地区：[1]重庆医科大学附属第一医院消化内科,重庆400016

出　　处：《重庆医科大学学报》2011年第2期159-162,共4页Journal of Chongqing Medical University

摘　　要：目的:探讨Hedgehog(Hh)信号通路对人胃癌SGC-7901细胞侵袭迁移的作用及其机制。方法:环靶明特异性阻断Hh信号通路后,Transwell小室和划痕实验检测SGC-7901细胞侵袭和迁移的能力;RT-PCR检测SGC-7901细胞中MMP-2和MMP-9 mRNA表达;细胞免疫化学检测SGC-7901细胞中MMP-2和MMP-9蛋白表达。结果:阻断Hh信号通路能显著抑制SGC-7901细胞的侵袭和迁移能力(P<0.05)。MMP-2和MMP-9的mRNA以及蛋白表达亦显著降低(P<0.05)。结论:Hh信号通路可能是通过调节MMP-2和MMP-9的表达促进SGC-7901细胞侵袭、迁移。Objective：To investigate the effect and mechanism of Hedgehog signaling pathway on invasion and migration of human gastric cancer cell line SGC-7901.Methods： After cyclopamine blocked Hh signaling pathway specifically,transwell chamber assay and wound healing assay were used to examine the capacities of invasion and migration of SGC-7901 cells respectively.Reverse transcriptase-polymerase chain reaction（RT-PCR） was used to detect the expression of MMP-2 and MMP-9 mRNA in SGC-7901 cells.Immunochemistry was used to detect the expression of MMP-2 and MMP-9 protein in SGC-7901 cells.Results： The capacities of invasion and migration of SGC-7901 cells could be significantly inhibited after blocking Hh signaling pathway（P0.05）.There was lower expression of MMP-2 and MMP-9 mRNA than the untreated group in SGC-7901 cells（P0.05）.The expression of MMP-2 and MMP-9 protein also remarkably decreased（P0.05）.Conclusion： Hedgehog signaling pathway may promote invasion and migration of SGC-7901 cells by regulating the expression of MMP-2 and MMP-9.

关 键 词：HEDGEHOG 胃癌 侵袭 迁移 MMP-2 MMP-9 

分 类 号：R735-2[医药卫生—肿瘤]