5-脂氧合酶抑制剂对人肝癌细胞株HepG2增殖和凋亡的影响  

Effect of Zileuton,a 5-lipoxygenase specific inhibitor on proliferation and apoptosis of human hepatoma cells(HepG2)

作　　者：邓君健[1] 徐细明[1] 郭红庭 向淼 吴耀贵[1] 张一桥[1] 戈伟[1] 宋启斌[1] 

机构地区：[1]武汉大学人民医院肿瘤中心,湖北武汉430060

出　　处：《现代肿瘤医学》2011年第3期410-413,共4页Journal of Modern Oncology

基　　金：国家自然科学基金资助项目(编号:81000998);湖北省自然科学基金资助项目(编号:2007ABA248);教育部博士点基金新教师资助项目(编号:20090141120003)

摘　　要：目的:研究5-脂氧合酶(5-lipoxygenase,5-Lox)在人肝癌细胞株HepG2中的表达,以及5-Lox抑制剂对人肝癌细胞株HepG2增殖、凋亡的影响。方法:应用免疫细胞化学染色及RT-PCR分别检测5-Lox的蛋白和mRNA在人肝癌细胞株HepG2中的表达,采用MTT法及流式细胞术检测5-Lox抑制剂齐留通(Zileuton)对人肝癌细胞株HepG2增殖、凋亡的影响。结果:5-Lox蛋白表达于肝癌细胞浆,局灶细胞核膜亦见阳性染色,RT-PCR可检测到肝癌细胞5-Lox的mRNA的表达。人肝癌细胞株HepG2给予不同浓度的齐留通处理24小时后,细胞存活率呈剂量依赖性下降;流式细胞术检测可见细胞凋亡率呈剂量依赖性升高。结论:人肝癌细胞株HepG2存在5-Lox的表达,5-Lox抑制剂齐留通可抑制人肝癌细胞株HepG2的增殖,诱导细胞凋亡。Objective：To examine the expression of 5-lipoxygenase in human hepatoma cell line（HepG2）,investigate the effect of its specific inhibitor Zileuton on cell proliferation and apoptosis.Methods：Immunochemical staining and RT-PCR were used to detect the expressions of 5-lipoxygenase protein and mRNA.Cell proliferation and apoptosis were determined by MTT assay and flow cytometry.Results：5-lipoxygenase protein was detected in cytoplasm,with focal nuclear membrane staining.The mRNA of 5-lipoxygenase was detected in HepG2 cells.After the treatment of Zileuton for 24 hours,Zileuton reduced cell proliferation and induced apoptosis in HepG2 cells in a concentration-dependent manner.Conclusion：5-lipoxygenase is expressed in human hepatoma cell line（HepG2）.Inhibition of 5-lipoxygenase by Zileuton reduces cell proliferation and induces apoptosis in a concentration-dependent manner.

关 键 词：人肝癌细胞株HEPG2 5-脂氧合酶 细胞凋亡 

分 类 号：R730.53[医药卫生—肿瘤] R735.7[医药卫生—临床医学]