丹参酮Ⅰ对人胃腺癌细胞SGC-7901增殖、凋亡的影响  

The effect of Tanshinone Ⅰ on proliferation and apoptosis of human gastric adenocarcinoma cell line SGC-7901

作　　者：周晓丽[1] 贾敏[2] 张永爱[1] 

机构地区：[1]西安医学院护理学院,陕西西安710021 [2]西安医学院科研中心,陕西西安710021

出　　处：《现代肿瘤医学》2011年第3期423-427,共5页Journal of Modern Oncology

基　　金：西安医学院青年扶持项目(编号:Q06)

摘　　要：目的:研究丹参酮I(Tan I)对SGC-7901人胃腺癌细胞增殖、凋亡的影响。方法:体外培养SGC-7901细胞,实验分为对照组,5-FU250μg/ml,Tan I 0.5μg/ml,Tan I 1μg/ml,Tan I 2μg/ml,Tan I 4μg/ml。MTT法检测SCG-7901细胞增殖情况;Hoechst33258/PI双荧光活染法观察凋亡细胞核形态学改变;流式细胞仪检测细胞周期分布,免疫细胞化学SABC法检测SGC-7901人胃腺癌细胞中Bcl-2的表达。结果:Tan I对胃腺癌细胞的生长有明显的抑制作用;Tan I作用后胃腺癌细胞表现为凋亡特征性的形态改变;Tan I可浓度依赖性引起G1期细胞数量增多,S期细胞减少;Tan I作用组胃腺癌细胞Bcl-2表达明显减少。结论:TanI对体外培养的SGC-7901人胃腺癌细胞的生长有明显的抑制作用,可通过促进其凋亡、影响细胞周期分布及抑制SGC-7901人胃腺癌细胞Bcl-2的表达发挥其抗肿瘤作用。Objective：To observe the effect of TanshinoneⅠ on proliferation and apoptosis of human gastric adenocarcinoma cell line SGC-7901 and its mechanis.Methods：The human gastric adenocarcinoma cell line SGC-7901was cultured in vitro and with TanshinoneⅠ at various concentrations.Cytotoxicity was valuated by MTT assay.Apoptosis-related alterations in morphology ascertained by cytochemical staining（Hoechst33258）.The cell cycle distribution and the apoptotic rate were investigated by flow cytometry.The expression of Bcl-2 was studied by immunocytochemistry.Results：The growth of SGC-7901 cells was inhibited significantly in a dose-dependent manner.SGC-7901 cell apoptosis with nuclear chromatin concentration and fragmentation as well as cell shrinkage and the formation of apoptotic bodies were observed by fluorescence inverse microscope.The percentage of cells in S phase increased in the SGC-7901 cells treated by TanshinoneⅠ.The expression of Bcl-2 of TanshinoneⅠ treated group was significantly lower than that of negative control group by t-test（P0.1）.Conclusion：The effect of TanshinoneⅠ on inducing apoptosis and inhibiting recurrence and metastasis of human gastric adenocarcinoma may be related with down-regulating Bcl-2 protein expression.

关 键 词：丹参酮I 胃腺癌 凋亡 BCL-2 

分 类 号：R730.53[医药卫生—肿瘤] R735.2[医药卫生—临床医学]