茶多酚对甲醛致人脐静脉内皮细胞损伤保护作用  

Effect of tea polyphenols on formaldehyde-induced damage of human umbilical vein endothelial cells

作　　者：李梓民[1] 邹云飞[2] 邹学敏[3] 袁萍[4] 贺栋梁[3] 胡余明[1] 

机构地区：[1]湖南省疾病预防控制中心毒理检验科,长沙410005 [2]皖南医学院 [3]南华大学 [4]长沙医学院衡阳校区

出　　处：《中国公共卫生》2011年第4期410-411,共2页Chinese Journal of Public Health

基　　金：湖南省教育厅科研基金(09C142)

摘　　要：目的观察茶多酚对甲醛致人脐静脉内皮细胞氧化损伤的影响。方法体外常规培养内皮细胞,实验设正常对照组、甲醛损伤组(0.1 mmol/L)、茶多酚组(2.5、5、10、20 mg/L)。其中茶多酚先与细胞预孵12 h后加入甲醛,置CO2培养箱培养4 h,检测细胞培养液中超氧化物歧化酶(SOD)活力及乳酸脱氢酶(LDH)、丙二醛(MDA)、一氧化氮和细胞中谷胱甘肽含量,免疫细胞化学法检测核转录因子(NF-κB)。结果 2.5、5、10、20 mg/L茶多酚组LDH、MDA含量分别为2 021.02、1 878.17、1 748.56、1 625.72 U/L和3.50、3.30、2.99、2.68 nmol/mL,均分别低于甲醛损伤组,差异有统计学意义(P<0.01);SOD与GSH活力分别为24.16、24.67、25.56、26.02 U/mL和0.334、0.364、0.445、0.449μmol/mg prot均高于甲醛损伤组(P<0.01);茶多酚各组NF-κB活力与甲醛组比较明显下降(P<0.01)。结论茶多酚可提高内皮细胞抗氧化酶活性,减轻甲醛所致氧化损伤。Objective To observe the effect of tea polyphenols（TP） on formaldehyde-induced oxidative damage of human umbilical vein endothelial cells. Methods Human umbilical vein endothelial cells were cultured with Dulbeccos modified Eagles medium in vitro and selected for exponential phase of growth for the experiment.Three groups were established as nomal group,formaldehyde injured group（0.1 mmol/L）,and TP protection groups（2.5,5,10,and 20 mg/L）,in which the tea polyphenols was firstly preincubated with the cells for 12hr before adding formaldehyde,followed by culturing for 4hr in CO2 incubator.The activity of superoxide dismutase（SOD） in cell culture solution,the level of lactate dehydrogenase（LDH）,malondialdehyde（MDA）,nitric oxide（NO） in the culture,and glutathione（GSH） and the activity of nuclear factor-kappa B（NF-κB） in the cells were detected. Results There were statistical differences in the levels of LDH,MDA,and NO between TP protection groups and formaldehyde-injured group,with the LDH levels of 2 021.02,1 878.17,1 748.56,and 1 625.72 U/L,MDA of 3.50,3.30,2.99,and 2.68 nmol/mL,and NO of 79.09,75.07,66.79,and 60.59 μmol/L for the TP group of 2.5,5,10,and 20 mg/L,respectively.Although the activity of NF-κB was significantly lower than that of formaldehyde group,the activity of SOD,GSH of the TP protection group were different from that of formaldehyde-injured group（P0.01）,with the SOD activity of 24.16,24.67,25.56,and 26.02 U/mL,GSH of 0.334,0.364,0.445,and 0.449μmol/mg,all higher than those of formaldehyde-injured group（SOD=23.72 U/ml and GSH=0.325 μmol/mg）. Conclusion Tea polyphenols can increase the activity of antioxidant enzymes in endothelial cells and reduce oxidative damage caused by formaldehyde.Tea polyphenols can lower generation of NO and ease intracellular lipid peroxidation by inhibiting the expression of NF-κB.

关 键 词：茶多酚 甲醛 人脐静脉内皮细胞 氧化损伤 核转录因子NF-ΚB 

分 类 号：R114[医药卫生—卫生毒理学]