参芎化瘀胶囊对脑缺血大鼠神经功能影响  

Effect of Shenxionghuayu capsule on neurological function after global cerebral ischemia/reperfusion in rats

作　　者：刘海娟[1] 赵雅宁[1] 李建民[1] 马素慧[1] 陈长香[1] 

机构地区：[1]华北煤炭医学院康复医学系,河北唐山063000

出　　处：《中国公共卫生》2011年第4期447-449,共3页Chinese Journal of Public Health

基　　金：河北省科技厅课题基金(2009276103D-3)

摘　　要：目的探讨参芎化瘀胶囊对脑缺血神经功能损伤的影响。方法 40只雄性SD大鼠分成假手术组、模型组、参芎化瘀胶囊高、低剂量组,采用改良的Pulsineli 4血管阻断(4-VO)法制作全脑缺血模型,脑缺血48 h,苏木精-伊红(HE)染色观察海马区神经细胞形态变化,原位缺口末端标记(TUNEL)法检测凋亡细胞,八臂迷宫法测试动物学习记忆功能,转棒法测试动物综合运动功能。结果与假手术组比较,模型组凋亡神经细胞数量(33.45±3.48)增多,动物进入迷宫总错误次数(8.94±3.78)、重复错误次数(7.32±2.80)、工作记忆错误次数(3.48±1.26)增多,综合运动评分(79.1±24.5)降低;与模型组比较,参芎化瘀胶囊组凋亡神经细胞数量(17.47±1.84)减少,动物进入迷宫总错误次数(3.00±1.80)、重复错误次数(2.10±0.85)降低,综合运动功能评分(208.9±32.5)提高。结论参芎化瘀胶囊可抑制全脑缺血大鼠神经细胞凋亡,改善脑缺血后神经功能损伤。Objective To investigate the effects of Shenxionghuayu capsule on neurological function after global cerebral ischemia/reperfusion. Methods Male Sprague-Dawley rats（n=40） were divided randomly into four groups：sham,model,low and high dose of Shenxionghuayu capsule treatment group.Global cerebral ischemia model was established by improved four-vessel occlusion of Pulsinelli′s method.Forty-eight hours after the operation,morphological changes in hippocampal region were observed by hetatoxylin eosin（HE） staining.The quantity of apoptotic cells was measured by terminal deoxynucleotidyl transfernase medicated nick end labeling（TUNEL） method.Learning and memory function（eight-arm maze） and behavioral tests（roding method） were conducted. Results Compared with the sham group,the numbers of neural apoptotic cells increased（33.45±3.48） in model group.The total error times（8.94±3.78）,the repeat error times（7.32±2.80）,and the reference memory error times（3.48±1.26） increased.The general movement ability（79.1±24.5） decreased in model group.Compared with the model group,the number of neural apoptotic cells decreased（17.47±1.84）;the total error time（3.00±1.80）and the repeat error time（2.10±0.85）decreased and the general movement ability（208.9±32.5） increased in Shenxionghuayu capsule group. Conclusion Shenxionghuayu capsule can dramatically improve neural function after cerebral ischemia/reperfusion injury,which may be related to the suppression of neuronal apoptosis in rats.

关 键 词：脑缺血 凋亡 神经功能 

分 类 号：R741[医药卫生—神经病学与精神病学]