牛磺酸对锌中毒小鼠免疫功能影响  

Effect of taurine on immune function in mice with zinc poisoning

作　　者：胡敏[1] 徐群英[1] 

机构地区：[1]南昌大学医学院公共卫生学院营养与食品卫生学教研室,江西南昌330006

出　　处：《中国公共卫生》2011年第4期449-450,共2页Chinese Journal of Public Health

基　　金：江西省教育厅资助项目(GJJ09118)

摘　　要：目的了解牛磺酸对锌中毒小鼠免疫因子及T淋巴细胞增殖功能影响。方法以不同浓度牛磺酸作用于锌中毒模型小鼠后,计算脏器指数,噻唑蓝(MTT)比色法测定T淋巴细胞增殖功能,酶联免疫吸附(ELISA)法测定白细胞介素-2(IL-2)、肿瘤坏死因子α(TNF-α)浓度。结果锌中毒模型组TNF-α、IL-2浓度分别为(9.02±1.34)、(1.411±0.082)pg/mL,T淋巴细胞增殖活性为(0.056 1±0.006),明显低于空白对照组(P<0.05),低剂量(200mg/kg)牛磺酸组TNF-α、IL-2分别为(15.92±2.32)、(1.928±0.103)pg/mL,T淋巴细胞增殖活性为(0.120 1±0.012),明显高于锌中毒模型组,差异有统计学意义(P<0.01)。结论牛磺酸可提高锌中毒小鼠外周血TNF-α、IL-2水平及促进T淋巴细胞增殖,对锌中毒小鼠免疫功能损伤有一定保护作用。Objective To study the effect of taurine on immune function and T-lymphocyte proliferation in mice with zinc poisoning. Methods The mice of zinc poisoning model were randomly fed with different dose of taurine.The organ index was calculated.The lymphocyte proliferation was examined with methyl thiazolyl tetrazolium（MTT） assay.The production of interleukin-2（IL-2） and tumor necrosis factor-α（TNF-α） in cells were measured with enzyme linked immunoabsorbent assay（ELISA）. Results The concentrations of TNF-α,IL-2 of the zinc poisoning control group were 9.02±1.34,1.411±0.082 and the proliferation power of T-lymphocytes of the zinc poisoning model group was 0.0561±0.006,all of which were significantly lower than those of the control group（P0.05 for all）.The concentrations of TNF-α,IL-2 of zinc poisoning group with low dose taurine（200mg/kg） were 15.92±2.32,1.928±0.103 and the proliferation power was 0.1201±0.012,significantly higher than those of zinc poisoning control group（P0.01）. Conclusion Taurine could increase concentrations of TNF-α,IL-2 and promote proliferation power of T-lymphocyte in mice with zinc poisoning.Taurine has protective effect on immune function impairment in mice with zinc poisoning.

关 键 词：牛磺酸 锌中毒 肿瘤坏死因子-Α 白细胞介素-2 淋巴细胞转化 

分 类 号：R114[医药卫生—卫生毒理学]