酒精性肝病中脂联素作用及其与HO-1关系  

Role of adiponectin and its relationship with heme oxygenase-1 in alcoholic liver disease

作　　者：王晓红[1] 曹琦[2] 汪思应[1] 胡成穆[2] 

机构地区：[1]安徽医科大学动物实验中心,安徽合肥230032 [2]安徽医科大学药学院

出　　处：《中国公共卫生》2011年第4期455-457,共3页Chinese Journal of Public Health

基　　金：安徽省高校优秀青年人才基金(2010SQRL065);安徽医科大学校科研基金(2008kj33)

摘　　要：目的探讨脂联素(adiponectin)mRNA及其蛋白水平在酒精性肝病发病过程中的变化及其与血红素氧化酶(heme oxygenase,HO)-1关系。方法建立大鼠酒精性肝病动物模型,检测血清中谷草转氨酶(ALT)、谷丙转氨酶(AST)和肿瘤坏死因子(TNF-α)及血浆中脂联素,肝匀浆中丙二醛(MDA)、谷胱甘肽(GSH-Px)和超氧化物歧化酶(SOD)的变化;采用RT-PCR法检测脂联素和HO-1 mRNA的表达,分析脂联素与HO-1 mRNA、SOD相关性。结果酒精组ALT、AST、TNF-α和MDA分别为(84.88±23.84)、(181.75±43.60)U/L,(18.40±0.87)pg/mL,(1.67±0.38)nmol/mgprot,而酒精肝炎组和酒精肝纤维化组中各指标水平明显增加(P<0.01);酒精组血浆脂联素水平为(0.16±0.03)ng/mL;GSH和SOD活性为(17.49±2.32)mg/mgprot,(22.63±0.26)U/mgprot,而酒精肝炎组和酒精肝纤维化组中各指标水平则明显降低(P<0.01);酒精组脂联素和HO-1 mRNA水平分别为(0.874±0.039)、(0.387±0.027),随着肝损伤程度加重,各指标呈现逐渐降低(P<0.01);脂联素与HO-1 mRNA、SOD具有相关性(R2=0.9662,R2=0.7403,P<0.01)。结论脂联素表达水平的下降与肝损伤程度有着一定联系,脂联素与HO-1可能构成一个新的抗炎途径参与了酒精性肝病的发病过程。Objective To investigate the expression of adiponectin protein and mRNA after chronic alcohol feeding as well as the relationship between heme oxygenase-1（HO-1）and adiponectin. Methods We established an animal model of alcoholic liver disease（ALD）.Meanwhile,serum alanine aminotransferase（ALT）and aspartate transaminase（AST）,plasma tumor necrosis factor（TNF）-α,glutathione（GSH）,superoxide dismutase（SOD）,and malonodialdehyde（MDA）in liver homogenate were detected.Adiponectin and HO-1mRNA were detected with RT-PCR.The corrlelations between adiponectin and HO-1mRNA,SOD were analyzed. Results The levels of ALT,AST in serum,TNF-α in plasma,and MDA concentration in liver homogenate were increased compared with those of the normal and control groups.Adiponectin in plasma,GSH and SOD activities in liver homogenate were decreased compared with those of the control and normal groups.Moreover,with the severity of the liver injury,the expression levels of adiponectin and HO-1 mRNA were decreased.The plasma level of adiponectin was positively correlaed with HO-1 mRNA expression level（R2=0.966 2,P0.01）and SOD activities（R2=0.740 3,P0.01）. Conclusion The expression level of adiponectin is significantly correlated with liver injury.Adiponectin and HO-1 constitute a new anti-inflammatory pathway which may be also involved in the pathogenesis of alcoholic liver disease process.

关 键 词：脂联素 酒精性肝病 血红素氧化酶-1 

分 类 号：R575[医药卫生—消化系统]