HSV-1感染对神经胶质瘤细胞NGF和BDNF表达变化的研究  

Study of NGF and BDNF expression in HSV-1 infected human glioma cells

作　　者：侯云[1] 王斌[1] 李玲[1] 胡明[1] 辛晓妮[1] 钱冬萌[1] 闫志勇[1] 赵巍[1] 宋旭霞[1] 

机构地区：[1]青岛大学医学院,青岛市医药生物技术重点实验室,266071

出　　处：《实用医学杂志》2011年第7期1132-1134,共3页The Journal of Practical Medicine

基　　金：国家自然科学基金项目(编号:30770105,81070501);“泰山学者”建设工程专项经费资助项目

摘　　要：目的:探讨单纯疱疹病毒1型(HSV-1)感染时神经胶质细胞内源性神经生长因子(NGF)和脑源性神经生长因子(BDNF)的表达变化。方法:采用RT-PCR法检测HSV-1糖蛋白D(gD)基因扩增,RT-PCR法和Westernblotting法检测正常培养和感染HSV-1的神经胶质瘤(U251)细胞的NGF和BDNF。结果:HSV-1可感染U251细胞;正常U251细胞可表达NGF和BDNF;HSV-1感染U251细胞后,NGF和BDNF在感染后第6小时达高峰,之后随感染时间延长逐渐降低。结论:HSV-1可诱导U251细胞中的NGF和BDNF表达异常。Objective To investigate endogenic nerve growth factor （NGF） and brain-derived neurotrophic factor （BDNF） expression in herpes simplex type- 1-virus （HSV- 1 ） -infected neuroglia cells. Methods U251 human glioma cells were cultured in RPMI-1640 medium and infected with HSV-1 in vitro to establish a cell model of viral infection. Establishment of HSV-l-infected U251 cells was identified by HSV-1 gD mRNA expression. NGF and BDNF expression was detected by RT-PCR and Western blotting, respectively, in normal U251 cells and HSV-l-infected U251 cells. Results HSV-1 could efficiently infect U251 cells. NGF and BDNF was identified to be expressed in U251. NGF and BDNF expression reached a maximum at 6 hours post HSV-1 infection, then NGF and BDNF expression decreased in time-dependent manner. Conclusions HSV-1 infection could dysregulate NGF and BDNF expression in the U251 cells.

关 键 词：神经胶质瘤 单纯疱疹病毒1型 神经生长因子 脑源性神经生长因子 

分 类 号：R739.4[医药卫生—肿瘤]