母鼠妊娠前慢性不可预见性应激对胎鼠大脑5-羟色胺1A受体表达的影响  

Effect of Pregestational Chronic Unpredictable Stress on Expression of 5-Hydroxytryptamine 1A Receptor of Brain in Fetal Rats

作　　者：黄月君[1] 史雪川[1] 许宏武[1] 杨汉华[1] 陈思红[1] 陈晓东[1] 

机构地区：[1]汕头大学医学院第二附属医院儿科,广东汕头515041

出　　处：《实用儿科临床杂志》2011年第6期404-407,共4页Journal of Applied Clinical Pediatrics

基　　金：广东省科技厅资助项目(2010B031600282)

摘　　要：目的观察母鼠妊娠前接受慢性不可预见性应激(CUS)后皮质醇(COR)水平改变对胎鼠大脑不同区域5-羟色胺1A(5-HT1A)受体表达的影响。方法 SD成年健康雌性大鼠20只随机分为正常对照母鼠组(8只)和CUS母鼠组(12只)。采用11种刺激方法对CUS母鼠组进行21 d CUS,构建CUS模型。造模前后CUS母鼠组和正常对照母鼠组进行开场实验及蔗糖水消耗实验,分析CUS对母鼠自主行为的影响。采用放射免疫法检测妊娠第20天母鼠血清COR水平,分析CUS对母鼠COR的影响。采用反转录-PCR(RT-PCR)法检测胎鼠大脑海马、额前皮质、下丘脑5-HT1A受体mRNA表达情况。结果 1.CUS母鼠组应激后开场实验的总路程、周边路程、中央路程和蔗糖水消耗实验的蔗糖水消耗量明显少于应激前及正常对照母鼠组。2.血清COR水平在正常母鼠组为(155.3±14.4)μg.L-1,CUS组为(190.2±19.8)μg.L-1,2组比较差异有统计学意义(P<0.01)。3.二组胎鼠的体质量和脑质量比较差异均无统计学意义。4.胎鼠海马中5-HT1A受体mRNA的表达量正常对照组为0.286±0.025,CUS组为0.228±0.132,2组比较差异有统计学意义(P<0.01);额前皮质中5-HT1A受体mRNA的表达量正常对照组为0.266±0.028,CUS组为0.249±0.027,2组比较差异有统计学意义(P<0.01);但2组胎鼠下丘脑中5-HT1A受体mRNA表达量比较差异无统计学意义(P>0.05)。结论母鼠妊娠前接受CUS,可引起胎鼠大脑各区域5-HT1A受体表达异常,其机制与母体血清中COR水平有关。实用儿科临床杂志,2011,26(6)Objective To investigate the effect of chronic unpredictable stress（CUS）before pregnancy on serum cortisol（COR）level of maternal rats and the expression of 5-hydroxytryptamine 1A（5-HT1A） of brain in fetal rats.MethodsAdult female SD rats were divided randomly into 2 groups： control group（n=8）and CUS model group（n=12）.All rats were tested in the open field test and sucrose intake test before and after CUS protocol.All maternal rats were sacrificed in embryonic day 20.Serum COR level of maternal rats was determined by using a standard radioimmunoassay kit.The expression levels of 5-HT1A receptor mRNA in hippocampus,hypothalamus and prefrontal cortex of fetal rats were studied by reverse transcription polymerase chain reaction（RT-PCR）.Results1.After CUS,the total distance,central distance and peripheral distance in the open field test and sucrose consumption in sucrose intake test of CUS group decreased compared with those before CUS and control group.2.CUS induced a significant increase in cortisol levels of the maternal rats（190.2±19.8） μg·L-1,compared with the control group[（155.3±14.4） μg·L-1].3.The body weight and brain weight between the fetal rats of 2 groups had no statistic significance.4.RT-PCR showed that the level of 5-HT1A receptor mRNA in the hippocampus of CUS group（0.228±0.132）less than in control group（0.286±0.025）,as well as in prefrontal cortex of CUS group（0.249±0.027）compared with control group（0.266±0.028）（Pa0.01）,but it had no effect in hypothalamus（P0.05）.ConclusionProgestational CUS reduced the expression of 5-HT1A receptor in corresponding brain of the fetus which related with COR.J Appl Clin Pediatr,2011,26（6）： 404-407

关 键 词：慢性不可预见性应激 皮质醇 胎鼠 5-羟色胺 大鼠 

分 类 号：R714.2[医药卫生—妇产科学]