基于mcrA基因的厌氧颗粒污泥产甲烷菌群分析  

Analysis of Methanogenic Community of Anaerobic Granular Sludge Based on mcrA Gene

作　　者：刘春[1] 李亮[1] 马俊科[1] 吴根[2] 杨景亮[1] 

机构地区：[1]河北科技大学环境科学与工程学院,石家庄050018 [2]科技部基础研究管理中心,北京100862

出　　处：《环境科学》2011年第4期1114-1119,共6页Environmental Science

基　　金：国家自然科学基金项目(50878072);河北省自然科学基金项目(E2008000694;E2009000709)

摘　　要：基于mcrA基因对阿维菌素废水处理工业化UASB厌氧颗粒污泥中产甲烷菌群进行分析,并与基于16S rRNA基因的产甲烷菌群分析结果进行比较.结果表明,基于2种目标基因PCR产物的DGGE图谱存在差异,但根据图谱计算所得产甲烷菌群Shannon多样性指数、Margalef丰富度指数和Berger-Parker优势度指数没有差异,表明基于2种目标基因的产甲烷菌群多样性分析基本一致.基于不同目标基因的优势产甲烷菌群系统发育种属的分析结果大体相似,产甲烷杆菌目和产甲烷八叠球菌目是厌氧颗粒污泥样品中的优势产甲烷种群;同时,分析结果的差异表明2种目标基因的检测特异性不完全相同.基于2种目标基因的产甲烷菌群FISH杂交区域具有很高的一致性,但杂交区域面积有所差异.基于mcrA基因FISH检测的产甲烷菌群平均相对丰度为24.25%±6.47%,低于基于16S rRNA基因FISH检测结果(33.42%±2.34%).以上结果表明,基于mcrA基因与基于16S rRNA基因的的产甲污泥菌群分析结果具有较高的相似度,mcrA基因可以作为16S rRNA基因的替代目标基因.The methanogenic community in anaerobic granular sludge from a full-scale UASB treating avernectin wastewater was analyzed based on mcrA gene,compared to 16S rRNA gene.The results indicated that the diversity indices of methanogenic community,including Shannon diversity index,Margalef richness index and Berger-Parker dominance index,were no difference between mcrA gene-based and 16S rRNA gene-based PCR products analysis by DGGE,although their DGGE band patterns were different,implying that the diversity analysis of methanogenic community based on mcrA genes was consistent with 16S rRNA gene.The phylogenetic analysis of dominant methanogenic populations based on these two target genes also showed resemble and Methanobacteriales and Methanosarcinales were determined to be the main orders of methanogenic populations in anaerobic granular sludge.On the other hand,the difference in phylogenetic analysis suggested simultaneously some group-specific of the two target genes.The hybridization of methanogenic community in FISH analysis based on two target genes was almost identical except a little different hybridization areas.The average relative abundance of methanogenic community was 24.25% ± 6.47% detected by FISH based on mcrA gene,lower than that based on 16S rRNA gene（33.42% ± 2.34%）.Then it could be concluded that the analysis of methanogenic community based on mcrA gene and 16S rRNA gene exhibited high resemblance and mcrA gene could used to be target gene for methanogenic community,as an alternative of 16S rRNA gene.

关 键 词：mcrA基因 厌氧颗粒污泥 产甲烷菌群 PCR-DGGE FISH 

分 类 号：X172[环境科学与工程—环境科学]