Occludin蛋白在重症急性胰腺炎小鼠肠上皮细胞的表达  

Expression of Occludin Protein in Intestinal Epithelial Cells in Mice Model of Severe Acute Pancreatitis

作　　者：吴善斌[1] 王学清[1] 郭鸿飞[1] 王孟春[1] 

机构地区：[1]中国医科大学附属盛京医院消化内科,沈阳110004

出　　处：《中国医科大学学报》2011年第3期217-219,223,共4页Journal of China Medical University

摘　　要：目的观察重症急性胰腺炎(SAP)小鼠肠黏膜上皮occludin蛋白的表达,探讨SAP时肠黏膜屏障功能改变。方法建立小鼠SAP模型,电镜下观察肠上皮超微结构,应用免疫组织化学法观察肠上皮occludin蛋白的表达。结果对照组电镜下肠上皮超微结构未见异常;实验组3 h无明显变化,其他时点可见肠上皮微绒毛稀疏、排列不整齐,部分断裂、脱落,细胞器受损,核内异染色质浓缩且边集,细胞连接增宽,24 h时改变最明显。免疫组织化学显示对照组occludin均匀一致地分布于肠上皮细胞连接处的顶端;实验组分布不均,occludin表达随时间推移逐渐降低,24 h最低。对照组与实验组比较,3 h时平均光密度值的差异无统计学意义,6、12和24 h时有统计学差异。结论 SAP小鼠早期即有肠黏膜屏障损伤,肠上皮细胞间连接增宽,其可能机制与肠上皮occludin蛋白的表达降低有关。Objective To observe the expression of occludin protein in intestinal epithelial cells in mice model of severe acute pancreatitis（SAP） and investigate the relationship between occludin protein expression and intestinal mucosal barrier injury.Methods The mice model of SAP was established.The ultrastructural changes in intestinal epithelial cells were observed under electron microscope,and the expression of occluding protein in intestinal epithelial cells was determined.Results The ultrastructure of intestinal epithelial cells was normal in control group.In experimental group,there was no ultrastructural change in intestinal epithelial cells at 3 hours,but the ultrastructural changes were found at 6,12,and 24 hours and were most prominent at 24 hours.We found that the microvilli were rare,disrupted,and even absent;the organelles were damaged;the heterochromatin was condensed along the nuclear membrane;and the cell junctions became widen.Occludin protein was uniformly distributed at the top of the junctions of intestinal epithelial cells in control group,but uneven distributed in experimental group.In experimental group,the expression of occludin decreased gradually and reached the minimum level at 24 hours.The average optical densities of occludin protein were 0.344 8±0.002 8 in control group and 0.343 1±0.002 0,0.342 3±0.001 9,0.339 4±0.001 5,and 0.309 5±0.002 7 in experimental group at 3,6,12,and 24 hours,respectively.There was no significant between-group difference in the average optical density at 3 hours,but the differences were significant at 6,12,and 24 hours（P 0.05）.Conclusion Intestinal mucosal barrier injury and widening of the junctions of intestinal epithelial cells occur in early stage of SAP in mice,and the decreased expression level of occludin may be involved in the possible mechanism.

关 键 词：重症急性胰腺炎 肠黏膜屏障 OCCLUDIN蛋白 

分 类 号：R57[医药卫生—消化系统]