银杏内酯、银杏黄酮对脑淋巴阻滞后SAH大鼠脑组织HO-1表达的影响  

Effects of ginkgolide and ginkgetin on expression of HO-1 in cerebral tissue of subarachnoid hemorrhage and blockade of cerebral lymphatic drainage in rats

作　　者：程子翠[1] 刘伟[1] 孙保亮[2] 薛秀荣[1] 杨明峰[2] 

机构地区：[1]泰安市中医医院脑病康复中心,山东泰安271000 [2]泰山医学院附属医院神经内科,山东泰安271000

出　　处：《中风与神经疾病杂志》2011年第3期196-199,共4页Journal of Apoplexy and Nervous Diseases

基　　金：国家自然科学基金(No.30570651;No.30670724;No.30770759);山东省教育厅科技计划项目(J05L10);山东省中医药科技发展计划项目(2005-231);山东省卫生系统高层次人才基金;泰安市大学生科技创新行动计划项目(2007D1041)

摘　　要：目的探讨银杏内酯、银杏黄酮对脑淋巴引流阻滞(CLB)后蛛网膜下腔出血(SAH)大鼠脑组织血红素氧合酶-1(HO-1)表达的影响。方法将48只Wistar大鼠分为正常对照组、SAH组、SAH+CLB组、SAH+CLB+生理盐水(NS)组、SAH+CLB+银杏内酯干预组(又分为低剂量、高剂量组)、SAH+CLB+银杏黄酮干预组(又分为低剂量、高剂量组)。用结扎颈部淋巴管、摘除淋巴结及注入自体动脉血法制作CLB和SAH大鼠模型。制作CLB模型后24h诱导SAH模型。用RT-PCR法检测大鼠脑组织HO-1mRNA的表达。结果各模型组大鼠脑组织HO-1mRNA表达明显高于正常对照组(均P<0.01),其中以SAH+CLB组、NS组表达进一步增强。银杏内酯和银杏黄酮组HO-1mRNA的表达水平明显高于SAH+CLB组、NS组(均P<0.01),其中两高剂量组表达又明显高于低剂量组(均P<0.01),两低剂量组大鼠脑组织HO-1mRNA的表达与SAH+CLB组及NS组差异无统计学意义(P>0.05)。结论 CLB可诱导SAH鼠脑组织HO-1表达水平明显增高,银杏内酯和银杏黄酮对之有促进作用,尤以高剂量组效果更明显。Objective To investigate the effects of ginkgolide and ginkgetin on expression of heine oxygenase-1 （ HO- 1 ）in cerebral tissue following subarachnoid hemorrhage（ SAH）and blockade of cerebral lymphatic drainage（CLB）. Methods The 48 Wistar rats were divided into normal control, SAH group, SAH ＋ CLB group, SAH ＋ CLB ＋ NS group, higher and lower dose of ginkgolide groups,higher and lower dose of ginkgetin groups. One day after the induction of CLB model, SAH model was produced. The expression of HO-1 mRNA in the brain were determined by RT-PCR. Results The expression of HO-1 mRNA in rat brain of the model group was significantly higher than the normal control group（ all P 〈0.01 ）. The expression of SAH ＋ CLB and NS group was more higher. The expression level of HO-1 mRNA in brain of ginkgolides and ginkgetin higher-dose group was significantly higher than the SAH ＋ CLB group and NS group（ P 〈 0.01 ）. The expression of two high-dose group also was significantly higher than that of low-dose groups（ all P 〈 0.01 ）. Conclusion HO-1 expression level of brain tissue was significantly increased after SAH ＋ CLB, Ginkgo ginkgolides and ginkgetin could en- hance the expression of HO-1 mRNA. Particularly,the effect of high-dose group was more obvious.

关 键 词：脑淋巴引流 蛛网膜下腔出血 血红素氧合酶1(HO-1) 银杏内酯 银杏黄酮 

分 类 号：R743.35[医药卫生—神经病学与精神病学]