超高压液相色谱-串联质谱法分析中药中甲胺磷残留物  

Determination of Methamidophos in Herbs by Ultra Performance Liquid Chromatography Combined with Quadrupole Mass Spectrometry

作　　者：肖竦[1] 

机构地区：[1]贵阳医学院基础医学院化学教研室,贵阳550004

出　　处：《中国实验方剂学杂志》2011年第7期72-73,共2页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:建立中药材中甲胺磷残留物的测定方法。方法:采用超高压液相色谱-串联质谱(UPLC-MS/MS)联用技术,采用Acquity UPLC BEHC18(2.1 mm×50 mm,1.7μm),乙腈-0.1%甲酸为流动相,梯度洗脱,以保留时间和离子对进行定性和定量,在ESI(+)和MRM监测模式下进行样品分析。结果:在0.5～10.0 mg.L-1,线性关系良好,回收率99.53%,RSD2.1%。结论:该法样品前处理简单、分析速度快、回收率和灵敏度高。Objective：To establish a method for determination of methamidophos in herbs.Method： The content of methamidophos in herbs was determined by UPLC-MS /MS.The chromatographic analysis was performed on an Acquity UPLC BEHC18（2.1 mm × 50 mm,1.7 μm） by a gradient elution employing of acetonitrile-0.1% formic acid.The analytes were detected using electrospray ionization tandem mass spectrometry with multiple reaction monitoring（MRM） in positive ion scan mode.Result： The linear range of methamidophos was 0.5-10.0 mg.L-1.The average recovery was 98.53% and RSD was 2.1%.Conclusion： The method was simple,sensitive,accurate and short of analytical cycle.

关 键 词：超高压液相色谱 质谱 甲胺磷 

分 类 号：R284.1[医药卫生—中药学]