特异性环氧合酶-2抑制剂SC236诱导肾癌细胞RCC-949生物学行为变化的实验研究  

Experimental Study on Biological Behavior Changes of Renal Cell Carcinoma RCC-949 Induced by Specific COX-2 Inhibitor SC236

作　　者：曾雪芳 柳宁 王太林 

机构地区：[1]湖南省岳阳市职业技术学院,湖南岳阳414000

出　　处：《实用预防医学》2011年第3期428-430,共3页Practical Preventive Medicine

摘　　要：目的探讨环氧合酶-2(COX-2)抑制剂SC236对肾癌细胞RCC-949生物学行为的影响。方法体外培养RCC-949细胞,不同浓度的SC236与之作用24、487、2 h,四甲基偶氮唑盐(MTT)法检测细胞的生长状况;50、150μmol/L SC236作用RCC-949细胞48 h,RT-PCR法检测COX-2 mRNA的表达;50、100μmol/L SC236作用RCC-949细胞24 h,ELISA法检测SC236细胞上清前列腺素(PGE2)的生成情况;100μmol/L SC236作用RCC-949细胞24 h后流式细胞仪检测细胞周期与凋亡情况。结果 SC236呈时间-剂量依赖性抑制RCC-949细胞的增殖;RT-PCR结果显示SC236能降低RCC-949细胞COX-2 mRNA的表达;不同浓度的SC236均能下调PEG2的释放,与对照组比较差异有统计学意义(P<0.05);流式细胞术结果表明不同浓度的SC236均能诱导RCC-949细胞发生凋亡,凋亡率同对照组比较差异有统计学意义(P<0.05)。结论特异性COX-2抑制剂SC236能通过COX-2依赖途径抑制肾癌细胞RCC-949增殖,能下调PEG2的释放,诱导了其发生凋亡。Objective To explore the effects of a specific COX-2 inhibitor SC236 on biological behavior of renal cell carcinoma RCC-949. Methods Various concentrations of SC236 were incubated with cultured RCC-949 in vitro for 24,48 and 72 hours,and the growth of RCC-949 was detected by the MTT method.RCC-949 was stimulated by 50 and 150 umol/L of SC236 for 48 hours,and then the expression of COX-2 mRNA was detected by RT-PCR.RCC-949 was stimulated by 50 and 100 umol/L of SC236 for 24 hours,and then PGE2 was detected by enzyme linked immunosorbent assay.RCC-949 was stimulated by 100 umol/L of SC236 for 24 hours,and then the cell cycle and apoptosis were detected by flow cytometer（FCM）. Results SC236 inhibited the proliferation of RCC-949 in a time-and dose-dependent manner and decreased the expression of COX-2 mRNA.Different concentrations of SC236 could restrain the liberation of PGE2 and induce RCC-949 to apoptosis, and there were statistically significant differences between experimental group and control group（P〈0.05）. Conclusions Specific COX-2 inhibitor SC236 can inhibit the proliferation of renal cell carcinoma（RCC-949）,regulate the delivery of PGE2 and induce RCC-949 to apoptosis.

关 键 词：RCC-949 SC236 COX-2 凋亡 

分 类 号：R-331[医药卫生]