HLA-B对喉癌Hep2细胞生物学行为的影响  

Effect of HLA-B on the biological behavior of laryngeal carcinoma Hep2 cell

作　　者：郭艳[1] 富伟能[2] 尚超[2] 钟鸣[1] 孙开来[2] 

机构地区：[1]中国医科大学口腔医学院,沈阳110007 [2]中国医科大学基础医学院

出　　处：《山东医药》2011年第11期21-22,33,共3页Shandong Medical Journal

基　　金：辽宁省自然科学基金资助项目(20092110)

摘　　要：目的探讨人类白细胞组织相容抗原(HLA)-B在喉癌发生、发展中的作用。方法采用RNA干涉技术下调Hep2细胞中HLA-B基因表达。用干涉效应最明显、转染siRNA的细胞作为观察组,转染PBS及无义siRNA的细胞作为对照1组及对照2组。采用流式细胞仪、MTT和细胞体外侵袭力分析方法检测各组细胞凋亡率、增殖情况、细胞周期及局部侵袭能力。结果 RNA干涉7 d观察组细胞凋亡率明显高于两对照组,细胞周期阻滞于S期;细胞增殖能力及局部侵袭能力明显强于两对照组,P均<0.05。结论 HLA-B表达降低或缺失在喉癌的发生、发展中起促进作用。Objective To investigate the effect of HLA-B on the occurrence and development of laryngeal carcinoma.Methods RNA interference technology was used to down-regulate the expression of human leukocyte antigen（HLA）-B in Hep2 cells.Cells transfected with siRNA at most effective interference were used as observed group,while cells with PBS and nonsense siRNA transfection as control group 1 and 2.Then flow cytometry,MTT assay and cell invasion in vitro were used to detect the biological effect of Hep2 cells induced by HLA-B RNA interference.Results The apoptotic rate of Hep2 cells in observed group at RNA interference 7th day was significantly higher than that in the two control groups,with S phase cell cycle blocked.The proliferation ability and invasion effect were also increased compared with the two control groups,all P〈0.05.Conclusion Decrease or loss of expression of HLA-B may improve the occurrence and development of laryngeal carcinoma.

关 键 词：喉肿瘤 喉癌 人类白细胞组织相容抗原-B RNA干涉 生物学行为 

分 类 号：R739.6[医药卫生—肿瘤]