KIR2DL1和KIR2DL3在炎症性肠病患者外周血和肠组织中的表达  

Expressions of KIR2DL1 and KIR2DL3 in Peripheral Blood and Intestinal Tissue in Patients with Inflammatory Bowel Disease

作　　者：黎文华[1] 郑萍[1] 刘占举[2] 

机构地区：[1]上海交通大学附属第一人民医院消化内科,200080 [2]同济大学附属第十人民医院胃肠内科

出　　处：《胃肠病学》2011年第2期82-85,共4页Chinese Journal of Gastroenterology

摘　　要：背景:KIR2DL1和KIR2DL3属抑制性杀伤细胞免疫球蛋白样受体家族成员,在一些免疫相关疾病中发挥重要作用。目前,KIR2DL1和KIR2DL3在炎症性肠病(IBD)中的研究还相对较少。目的:探讨KIR2DL1和KIR2DL3在IBD患者外周血和肠组织中的表达及其临床意义。方法:收集111例溃疡性结肠炎(UC)患者、101例克罗恩病(CD)患者和110例健康志愿者。以聚合酶链反应-序列特异性引物(PCR-SSP)法检测外周血中KIR2DL1和KIR2DL3表达,并分析其与患者临床特征的关系。以免疫组化染色检测肠组织中KIR2DL1蛋白表达。结果:UC组外周血KIR2DL1和KIR2DL3表型频率均显著低于正常对照组(P<0.05);CD组KIR2DL1表型频率显著低于正常对照组(P=0.026),但两组KIR2DL3表型频率无明显差异(P>0.05)。KIR2DL1和KIR2DL3表型频率与UC和CD患者的疾病活动性、病情严重程度和病变部位均无关。UC和CD患者肠组织中KIR2DL1蛋白表达均明显低于对照组(P<0.001)。结论:IBD患者中存在KIR2DL1基因和蛋白的低表达,提示其在IBD的免疫发病机制中可能起重要作用。Background： KIR2DL1 and KIR2DL3 are members of inhibitory killer cell immunoglobulin-like receptor family, which play important roles in some immune-associated diseases. However, there are only few studies on KIR2DL1 and KIR2DL3 expressions in inflammatory bowel disease 0BD）. Aims： To investigate the expressions and clinical significanees of KIR2DL1 and KIR2DL3 in peripheral blood and intestinal tissue in patients with IBD. Methods： One hundred and eleven patients with ulcerative colitis （UC）, 101 Crohn＇s disease （CD） and 110 healthy volunteers were enrolled. Polymerase chain reaction-sequence specific primer （PCR-SSP） was used to determine the expressions of KIR2DL1 and KIR2DL3 in peripheral blood, and their relationships with clinical characteristics were analyzed. Protein expression of KIR2DL1 in intestinal tissue was determined by immunohistochemical staining. Results： Compared with normal controls, phenotypic frequencies of KIR2DL1 and KIR2DL3 in UC patients were significantly decreased （P〈0.05）; phenotypic frequency of KIR2DL1 was significantly decreased （P=0.026）, while no significant difference was found in phenotypic frequency of KIR2DL3 in CD patients （P〉0.05）. Phenotypic frequencies of KIR2DL1 and KIR2DL3 were not related with disease activity, severity of disease and site of lesions in UC and CD patients. Protein expression of KIR2DL1 in intestinal tissue in IBD patients was significantly lower than that in controls （P〈0.001）. Conclusions： Gene and protein expressions of KIR2DL1 are low in IBD patients, which suggest that KIR2DL1 may play an important role in the immunopathogenesis of IBD.

关 键 词：结肠炎 溃疡性 CROHN病 杀伤细胞免疫球蛋白样受体 杀伤细胞 天然 

分 类 号：R574[医药卫生—消化系统]