IL-1β、IL-10在大鼠溃疡性结肠炎模型中的表达  

Expression of IL-1β and IL-10 mRNA in Rats with Experimental Ulcerative Colitis

作　　者：李进[1] 杨锐[1] 邓豫[1] 蔡少鑫[1] 曹小年[1] 李小兰[1] 陶德定[1] 胡俊波[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院分子医学中心,武汉430030

出　　处：《南昌大学学报（医学版）》2010年第12期41-44,F0003,共5页Journal of Nanchang University：Medical Sciences

基　　金：国家自然科学基金(30872472;30973496;30800569);湖北省自然科学基金(2008CDB174;2009CDB239)

摘　　要：目的探讨促炎因子IL-1β和抑炎细胞因子IL-10在三硝基苯磺酸(TNBS)/乙醇诱导的溃疡性结肠炎(ul-cerative colitis,UC)大鼠模型结肠组织中的表达,探讨其在该模型中的作用和意义。方法将64只雄性Wistar大鼠按随机数字表法分为正常对照组与UC模型组,每组32只。正常对照组不造模,UC模型组用三硝基苯磺酸(TNBS)/乙醇溶液灌肠诱导UC模型。观察2组实验大鼠第1、3、7、14天体质量变化、组织病理学改变及结肠组织髓过氧化物酶(MPO)活性。实时荧光定量PCR检测2组大鼠结肠组织细胞因子IL-1β、IL-10 mRNA的表达。结果与正常对照组相比,UC模型组第1、3、7、14天大鼠结肠组织学评分均明显升高(P<0.01),结肠组织MPO活性明显增高(P<0.01),IL-1β,IL-10 mRNA相对于GAPDH的表达量均明显增高(P<0.01)。结论促炎细胞因子IL-1β与抑炎细胞因子IL-10的失衡在TNBS诱导的UC模型大鼠发病中起重要作用。Objective To study the expression of interleukin 1β（IL-1β） and interleukin 10（IL-l0） induced by 2,4,6 trinitrobenzenesulfonic acid（TNBS）/ethanol in colonic tissue in ulcerative colitis（UC） rats,and to explore its effect and significance in pathogenesis of ulcerative colitis.Methods Sixty-four male Wistar rats were randomized into control group and UC model group with 32 rats in each group.UC was induced by intrarectal administration of TNBS（100 mL·kg-1 in 50% ethanol） in model group.Animals were killed at day 1,3,7 and 14,respectively.Body weight loss and histological changes in the colon were observed.The activity of myeloperoxidase（MPO） was measured by biochemical method,and the expression of IL-1β and IL-10 mRNA in colonic tissues was tested by real-time PCR.Results Compared with the control group,histological score increased in model group at day 1,3,7 and 14（P0.01）.In addition,the activity of MPO and the expression of IL-1β and IL-10mRNA in model group were higher than those in control group（P0.01）.Conclusion The imbalance between proinflammatory cytokine（IL-1β）and anti-inflammatory cytokine（IL-10）plays an important role in the pathogenesis of TNBS-induced ulcerative colitis.

关 键 词：溃疡性结肠炎 白细胞介素-1Β 白细胞介素-10 三硝基苯磺酸 动物 实验 大鼠 

分 类 号：R-332[医药卫生]