罗格列酮对人结肠癌Lovo细胞增殖的抑制作用及其机制  

Inhibitory Effect of Rosiglitazone on Proliferation of Human Colon Cancer Lovo Cells and Relevant Mechanism

作　　者：吴柯[1] 何百成[1] 周岐新[1] 

机构地区：[1]重庆医科大学药学院药理教研室重庆市生物化学与分子药理学重点实验室,重庆400016

出　　处：《中国生物制品学杂志》2011年第3期276-279,共4页Chinese Journal of Biologicals

基　　金：国家自然科学基金资助项目(30572353)

摘　　要：目的探讨罗格列酮对人结肠癌Lovo细胞增殖的抑制作用及其可能的作用机制。方法人结肠癌Lovo细胞分别经不同浓度(10-6、10-5、10-4和10-3 mol/L)的罗格列酮和美洛昔康作用6、12和24 h后,采用MTT法检测细胞的增殖活性,RT-PCR法检测罗格列酮处理24 h的细胞COX-2基因mRNA的转录水平,Western blot法检测细胞COX-2蛋白的表达水平。结果罗格列酮浓度大于10-5 mol/L,美洛昔康浓度大于10-4 mol/L均可明显抑制Lovo细胞增殖,且呈浓度和时间依赖性;罗格列酮可明显降低Lovo细胞COX-2基因mRNA和蛋白的表达水平,且均呈浓度依赖性。结论罗格列酮能抑制人结肠癌Lovo细胞增殖,其作用机制与抑制细胞COX-2的表达有关。Objective To investigate the inhibitory effect of rosiglitazone（Ros） on proliferation of human colon cancer Lovo cells as well as the relevant mechanism.Methods Lovo cells were treated with various concentrations（10-6、10-5、10-4 and 10-3 mol / L） of Ros and Meloxicam（Mel） for 6,12 and 24 h separately,and determined for proliferative activity by MTT method.The cells 24 h after treatment with Ros were determined for transcription level of COX-2 mRNA by RT-PCR,and for expression level of cyclooxygenase-2（COX-2） protein by Western blot.Results The Ros at concentrations of more than 10-5 mol / L and the Mel at concentrations of more than 10-4 mol / L showed significantly dose and time-dependent inhibitory effect on the proliferation of Lovo cells.Ros decreased the expression levels of COX-2 mRNA and protein significantly,both in dose-dependent modes.Conclusion Ros inhibited the proliferation of Lovo cells by a mechanism associated with the inhibition of COX-2 expression.

关 键 词：罗格列酮 结肠肿瘤 环氧化酶-2 

分 类 号：R735.35[医药卫生—肿瘤]