激活素A及激活素结合蛋白在急性酒精性肝损伤小鼠肝组织中的表达  

Expression of Activin A and Activin-binding Protein in Liver Tissues of Mice with Acute Alcohol-induced Liver Lesion

作　　者：于昉[1] 王轶楠[1] 杨清[2] 孟小丹[1] 孙洋[1] 台桂香[1] 柳忠辉[1] 

机构地区：[1]吉林大学白求恩医学院免疫学系,长春130021 [2]吉林大学第一医院输血科,长春130031

出　　处：《中国生物制品学杂志》2011年第3期285-287,291,共4页Chinese Journal of Biologicals

基　　金：国家自然科学基金(30771957);吉林省科技厅(20060928-01);吉林省卫生厅(2006z010)

摘　　要：目的探讨激活素A(Activin A)及激活素结合蛋白(Follistatin,FS)在急性酒精性肝损伤小鼠肝组织中的表达。方法通过1次/12 h给予小鼠5 g/Kg酒精连续灌胃3次,复制急性酒精性肝损伤小鼠模型,采用实时定量RT-PCR检测小鼠肝组织Activin A及FS mRNA表达水平,免疫组织化学染色观察小鼠肝组织Activin A及FS蛋白的表达水平。结果急性酒精性肝损伤小鼠肝组织Activin A mRNA和蛋白表达水平均显著高于对照组(P<0.01);FS mRNA和蛋白表达水平与对照组比较,差异均无统计学意义(P>0.05)。结论急性酒精性肝损伤小鼠肝组织Activin A-FS表达失衡,以Activin A升高为主,提示Activin A-FS系统失衡可能与酒精性肝损伤有关。Objective To investigate the expression of activin A and activin-binding protein（Follistatin,FS） in liver tissues of mice with acute alcohol-induced liver lesion.Methods Mice were administered with alcohol by gavage at a dosage of 5 g / kg,once 12 h for 3 times,to copy the mouse model of acute alcohol-induced liver lesion.The transcription levels of activin A and FS mRNAs in liver tissues of mice were determined by real-time quantitative RT-PCR,while the expression levels of activin A and FS by immunohistochemical staining.Results Compared with those in control group,the mRNA and protein levels of activin A in liver tissues of model mice were significantly high（P 0.01）,while those of FS showed significant difference（P 0.05）.Conclusion The expression of activin A-FS in liver tissues of mice with acute alcohol-induced liver lesion was unbalanced,which was mainly expressed as increased activin A,indicating that the unbalance of activin A-FS system might be associated with alcohol-induced liver lesion.

关 键 词：激活素类 激活素结合蛋白 肝疾病 酒精性 

分 类 号：R575[医药卫生—消化系统]