益气活血法对兔视网膜激光损伤后bFGF和GFAP表达的影响  

Effect of yiqihuoxuefa on expression of bFGF and GFAP in rabbit retina injured by laser photocoagulation

作　　者：江运长[1] 彭清华[2] 杨霞[3] 

机构地区：[1]湘南学院附属医院眼科,湖南省郴州市423000 [2]湖南中医药大学第一附属医院眼科,湖南省长沙市410007 [3]湖北航天医院眼科,湖北省孝感市432000

出　　处：《眼科新进展》2011年第3期220-222,227,共4页Recent Advances in Ophthalmology

基　　金：湖南省自然科学基金资助(编号:06JJ0056)~~

摘　　要：目的探讨益气活血法对兔视网膜激光光凝损伤的防治作用和相应机理。方法 50只健康有色家兔随机分为正常对照组、模型对照组、黄芪组、蛴螬组、益气活血组。黄芪组每天给予黄芪水煎液5mL.kg-1灌胃;蛴螬组每天给予蛴螬水提液5mL.kg-1灌胃;益气活血组每天给予益气活血水煎液5mL.kg-1灌胃;正常对照组、模型对照组给予等量生理盐水灌胃,每天一次。于造模后第7天、第14天分两批处死实验兔,行眼球摘除术,视网膜组织固定后,石蜡包埋,作病理组织学切片,采用免疫组织化学法观察视网膜碱性成纤维细胞生长因子(basic fibroblast growthfactor,bFGF)及胶质纤维酸性蛋白(glial fibrillary acidic protein,GFAP)的表达。结果 术后第7天,正常对照组、模型对照组、黄芪组、蛴螬组及益气活血组视网膜bFGF表达的灰度值分别为84.54±15.16、134.00±15.94、128.72±12.47、128.94±9.50及105.16±5.17,GFAP表达的灰度值分别为141.72±12.30、91.44±13.39、90.22±15.59、84.14±25.04及119.08±6.92;术后第14天,bFGF表达的灰度值分别为81.30±18.11、136.00±14.41、122.00±6.64、126.98±7.97及102.12±7.79,GFAP表达的灰度值分别为136.68±7.61、92.30±11.79、92.18±15.28、86.66±22.62及117.66±5.30。益气活血组与其他激光损伤组比较,能显著增强bFGF的表达,降低视网膜GFAP的表达,差异均有统计学意义(均为P<0.05)。结论 益气活血法可以通过增强视网膜组织中bFGF的表达、抑制GFAP的表达,促进眼底激光视网膜损伤的修复,对视网膜激光光凝损伤有一定的防治作用。Objective To discuss the protective effect and mechanism of yiqihuoxuefa on rabbit retina injured by laser photocoagulation.Methods Fifty chinchilla rabbits were divided randomly into normal group,model group,astragalus group,grub group and yiqihuoxuefang group.Astragalus at dose of 5 mL·kg-1 was given in astragalus group once a day;Grub at dose of 5 mL·kg-1 was given in grub group;Yiqihuoxuefang at dose of 5 mL·kg-1 was given in yiqihuoxuefang group;Same volume PBS was given in normal group and model group,once a day.At the 7th and 14th day after laser photocoagulation,the eyes were enucleated,fixed and cut into paraffin sections,respectively.Immunohistochemical examination was used to detect the expression of basic fibroblast growth factor（bFGF） and glial fibrillary acidic protein（GFAP）.Results The gray values of bFGF expression in normal group,model group,astragalus group,grub group and yiqihuoxuefang group at the 7th day after laser photocoagulation were 84.54±15.16,134.00±15.94,128.72±12.47,128.94±9.50,105.16±5.17,respectively,and the gray values of GFAP expression were 141.72±12.30,91.44±13.39,90.22±15.59,84.14±25.04,119.08±6.92,respectively.The gray values of bFGF expression in normal group,model group,astragalus group,grub group and yiqihuoxuefang group at the 14th day after laser photocoagulation were 81.30±18.11,136.00±14.41,122.00±6.64,126.98±7.97,102.12±7.79,respectively,and the gray values of GFAP expression were 136.68±7.61,92.30±11.79,92.18±15.28,86.66±22.62,117.66±5.30,respectively.Compared with the other injury groups,the expression of bFGF was increased and GFAP was decreased evidently in yiqihuoxuefang group,there were statistical differences（All P0.05）.Conclusion Yiqihuoxuefa can enhance the expression of bFGF and inhibit the expression of GFAP in retina,promote the reparation and have a protective effect on retina injury induced by laser photocoagulation.

关 键 词：益气活血法 激光光凝 视网膜损伤 碱性成纤维细胞生长因子 胶质纤维酸性蛋白 

分 类 号：Q959.836[生物学—动物学] R774[医药卫生—眼科]