兰州地区呼吸道感染患儿中人类博卡病毒1～3型检测与临床研究  

Detection and clinical characteristics analysis of human Bocavirus 1-3 in children for acute respiratory infection in Lanzhou area

作　　者：曹长青[1] 李宇宁[1] 金玉[2] 谢志萍[3] 高寒春[3] 周琼华[4] 高小倩[1] 张亚婷[1] 张健[1] 段招军[3] 

机构地区：[1]兰州大学第一医院儿科,730000 [2]南京医科大学附属南京儿童医院 [3]中国疾病预防控制中心病毒病预防控制研究所 [4]湖南省人民医院

出　　处：《中华实验和临床病毒学杂志》2011年第1期5-7,共3页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的了解兰州地区急性呼吸道感染患儿中人博卡病毒1—3型（HBoV1～3）感染的临床及分子流行病学特征。方法收集兰州大学第一医院2009年12月至2010年11月急性呼吸道感染患儿鼻咽分泌物及咽拭子标本524份，用：巢氏PCR扩增人博卡病毒（HBoV）NS1片段，检测HBoV1～3；同时PCR检测常见呼吸道病毒。结果524份标本中检出HBoV43例，检出率为8．2％，仅次于鼻病毒、呼吸道合胞病毒、副流感病毒3型；混合感染率为69．8％。其中人博卡病毒1型（HBoV1）在下呼吸道感染中检出率显著高于上呼吸道感染的检出率；2例人博卡病毒2型（HBoV2）患儿都出现胃肠道症状，与标准株GU048662．1的核苷酸同源性分别为99％和100％；1例人博卡病毒3型（HBoV3）与标准株HM132056．1的核苷酸同源性为99％。结论本地区儿童急性呼吸道感染中博卡病毒感染以HBoV1为主，首次检出HBoV3；人博卡病毒与其他病毒有较高的合并感染。人博卡病毒是本地区儿童急性呼吸道感染的重要病原之一。Objective To study the clinical and molecular epidemiology characteristics of human Bocavirus 1-3 （HBoV1-3） in children for acute respiratory infection in Lauzhou area. Methods Nasopharyngeal aspiration samples and throat swabs were collected from 524 children with ARTI at the First Hospital of Lanzhou University,Gansu Province, China,between December 2009 and November 2010. Nested PCR was employed to screening HBoV1-3, which amplified a 518-bp fragment of the partial NS1 gene. Furthermore, a standard reverse transcription-PCR was used to screen for other common respiratory viruses. Results The overall frequency of HBoV was 8.2% （43/524） , lining up behind human rhinovirus, RSV, parainfluenza virus 3. Thirty of the HBoV-postive children （69.8%） were co-infected with other respiratory viruses. The prevalence of HBoV1 in ALRTI was obviously higher than that in AURI. The 2 HBoV2 NS1 sequences shared 99% and 100% nucleotide sequence identity with HBoV2 strain CU47TH respectively. Two cases of HBoV2 postive children appears gastrointestinal symptoms. The one HBoV3 NS1 sequences shared 99% nucleotide sequence identity with HBoV3 isolate 46-BJ07. Conclusion The HBoV3 was detected at the first time in lanzhou area. HBoV1-3 infection exists in children with acute respiratory tract infections in Lanzhou region, HBoV1 were dominant. The mixed infection rate was higher.

关 键 词：呼吸道感染 博卡病毒1~3型 人 聚合酶链反应 

分 类 号：R72[医药卫生—儿科]