南京地区儿童人偏肺病毒感染的流行病学及临床特征分析  

Molecular epideiological and clinical feature of human metapneumovirus in children with acute respiratory tract infection in Nanjing City

作　　者：金玉[1] 张静[2] 赵德育[1] 谢志萍[3] 高寒春[3] 高小倩[2] 张亚婷[2] 段招军[3] 

机构地区：[1]南京医科大学附属南京儿童医院,210008 [2]兰州大学第一医院儿科 [3]中国疾病预防控制中心病毒病预防控制所

出　　处：《中华实验和临床病毒学杂志》2011年第1期11-13,共3页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的了解南京地区儿童人偏肺病毒（hMPV）感染的流行病学特点及临床特征。方法收集2009年8月至2010年7月南京医科大学附属南京儿童医院住院及门诊呼吸道感染患儿的鼻咽抽吸物（NPA）及咽拭子（NPS）共642例，采用逆转录聚合酶链反应法（RT—PCR）检测hMPVM基因，将阳性PCR扩增产物进行测序、同源性和进化分析。结果642例标本中共检出hMPV阳性扩增产物35份，检出率为5．5％。系统进化分析显示南京地区hMPVB1型占51．4％，A2b型占31．4％。hMPV的发病高峰在4月份。其致呼吸道感染以1岁以内多见（71．4％）。35例hMPV感染患儿中有15例（42．8％）存在混合感染，其中与HRV的混合感染检出率最高。临床诊断以肺炎（17例，48．6％）最为常见。结论人偏肺病毒是南京地区儿童急性呼吸道感染的重要病原之一，该年度其优势流行型别为B1型，南京地区A、B两型hMPV感染患儿临床特征无明显差异。Objective To investigate the molecular epideiological and clinical feature of human metapneumovirus in children with acute respiratory tract infection in Nanjing city, China. Method Nasopharyngeal aspirates and nasopharyngeal swab were taken from 642 outpatients or hospitalized pediatric patients with acute at the Children Hospital of Nanjing, Jiangsu Province, China, between August 2009 and July 2010. Respiratory speciments were tested for the M gene of hMPV by reverse-transcription polymerase chain reaction （RT-PCR）. All RT-PCR positive products were sequenced and phlogenetic analysis was conducted. Result hMPV was detected in 35 （5.5%） of the 642 children. Phylogenetic analysis revealed that 51.4% of the hMPV were B1, 31.4% were A2b. The peak of the positive rate was in April. The majority of the hMPV-positive patients（71.4% ） were 0-1 years old. Of the 35 hMPV-positive patients, 15 （42.8%） were co-infected with other respiratory viruses, and human rhinovirus （HRV） were the most common additional respiratory virus. The most common clinical diagnosis was pneumonia （48.6%）. Conclusion Human metapneumovirus is an important pathogen of acute respiratory tract infection in children in Nanjing city. The subtype B1 was the predominating lineage in 2009-2010 in Nanjing city. No significant differences were found for clinical characteristics between genotype A and genotype B human metapneumovirus infection in children in Nanjing.

关 键 词：人偏肺病毒 呼吸道感染 流行病学 儿童 学龄前 

分 类 号：R72[医药卫生—儿科]