第10号染色体上磷酸酶和张力蛋白同源缺失的基因对人卵巢癌A2780细胞侵袭和迁移能力的影响及相关机制  

Effects of PTEN gene on invasion and migration of ovarian cancer cell line A2780 and related mechanisms

作　　者：吴卉娟[1] 郝权[1] 王珂[1] 刘文欣[1] 马丁[2] 

机构地区：[1]天津医科大学附属肿瘤医院妇瘤科天津市肿瘤防治重点实验室,300060 [2]华中科技大学同济医学院妇产科

出　　处：《中华肿瘤杂志》2011年第3期165-168,共4页Chinese Journal of Oncology

基　　金：国家自然科学基金（30901742）;国家重点基础研究发展计划（2009CB521800）

摘　　要：目的探讨第10号染色体上磷酸酶和张力蛋白同源缺失的基因（PTEN）对人卵巢癌A2780细胞侵袭和迁移能力的影响及相关机制。方法将携带外源性人PTEN基因的野生型质粒（WT—PTEN）和磷酸酶域突变型质粒（C124A—PTEN）转入人卵巢癌细胞株A2780中，利用Transwell小室法和划痕愈合实验检测转染前后细胞侵袭和迁移能力的变化，应用Western blot检测各组细胞PTEN及其下游相关蛋白表达的变化。以空载体质粒和未转染细胞作为对照。结果细胞侵袭实验显示，WT-PTEN／A2780、C124A—PTEN／A2780、GFP／A2780和A2780细胞穿透Matrigel胶的细胞数，分别为（24．3±2．5）个、（43．7±3．8）个、（44．7±2．1）个和（45．0±3．0）个，WT-PTEN／A2780细胞的穿膜数明显少于其他各组（P〈0．05）。划痕愈合实验显示，WT—PTEN／A2780、C124A-PTEN／A2780、GFP／A2780和A2780细胞的迁移距离分别为（54．1±3．7）μm、（78．7±3．4）μm、（78．1±3．1）μm和（76．8±3．5）μm，WT-PTEN／A2780细胞的迁移速度明显慢于C124A—PTEN／A2780、GFP／A2780和A2780细胞（P〈0．05）。WT-PTEN／A2780细胞中基质金属蛋白酶9（MMP-9）蛋白的表达明显低于C124A．PTEN／A2780、GFP／A2780和A2780细胞（P〈0．05）。结论野生型PTEN可有效抑制A2780细胞的侵袭和迁移，该抑制作用与A2780细胞中MMP-9表达下调有关。Objective To evaluate the effects of PTEN on invasive and migration ability of human ovarian cancer cell line A2780 and related mechanisms. Methods The plasmid including WT-PTEN and mutant PTEN were transferred into A2780 cells. The invasive and migration ability were measured before and after transfection by transwell chamber and wound-healing assays. The expression of PTEN protein and related proteins in the cells were detected by Western blot analysis. Empty plasmid-transfected 3.2780 and normal A2780 cells were used as control （ the different four groups were named as WT-PTEN/A2780, C124A-PTEN/A2780, GFP/A2780 and A2780 ）. Results The number of penetrating cells was significantly less in WT-PTEN/A2780 cells（24.3 ±2.5 ） than those in C124A-FFEN/A2780, GFP/A2780 andA2780 cells （43.7±3.8, 44.7 ±2. 1 and 45.0±3.0 ） （P〈0.05）. The migration distance was markedly shorter in WT-PTEN/A2780 cells （54. 1± 3.7） than those in C124A-PTEN/A2780, GFP/A2780 andA2780eells （78.7±3.4, 78.1 ±3.1 and76.8±3.5）（P〈0. 05）. Conclusions Transfectionwith PTEN may suppress the invasive and migration ability of ovarian cancer cell line A2780 depending on its phosphatase activity, and the suppressive effect may be due to the down-regulation of MMP-9 in the cancer cells.

关 键 词：卵巢肿瘤 第10号染色体上磷酸酶和张力蛋白同源缺失的基因 基质金属蛋白酶9 侵袭 迁移 

分 类 号：R737.31[医药卫生—肿瘤]