Peroxiredoxin I蛋白在肝癌门静脉癌栓组织中的表达及其临床意义  

Expression and clinical significance of peroxiredoxin I in hepatocellular carcinoma with portal vein tumor thrombosis

作　　者：郭卫星[1] 薛捷[1] 李楠[1] 冯宇雄[2] 石洁[1] 胡华生[1] 谢东[2] 程树群[1] 吴孟超[1] 

机构地区：[1]第二军医大学东方肝胆外科医院肿瘤综合治疗科,上海200438 [2]中国科学院上海生命科学研究院营养研究所,上海

出　　处：《中华肝胆外科杂志》2011年第3期216-218,共3页Chinese Journal of Hepatobiliary Surgery

基　　金：基金项目：上海市科委基础研究重点资助项目（07JC14066）;上海市教育发展基金会曙光学者培养计划资助项目（2005）

摘　　要：目的探讨抗氧化还原蛋白1（PeroxiredoxinI，PrxI）在肝细胞癌合并门静脉癌栓组织中的表达及其与肝癌门静脉癌栓患者术后复发的关系。方法应用免疫组化法和蛋白印迹法检测40例肝细胞癌合并门静脉癌栓组织中PrxI蛋白的表达。于大鼠肝癌模型进一步观察PrxI蛋白在肝癌门静脉癌栓形成中的病理变化，运用免疫组化法检测在癌栓形成过程中PrxI蛋白其阳性表达率的改变。临床随访40例肝癌合并门静脉癌栓患者术后肿瘤首次复发时间与PrxI蛋白阳性表达的关系。结果免疫组化显示肝癌原发灶组织的PrxI蛋白表达阳性率明显高于癌栓组织的表达阳性率（70％比15％，P〈0．05），蛋白印迹结果显示Prx I蛋白在组织中的表达与免疫组化具有相同趋势，条带光度平均值在原发灶和癌栓组分别为1534．2和735．6（P〈0．01）。大鼠肝癌模型汇管区肝癌组织在4、8、12、16、20、24周时，PrX I蛋白阳性表达率分别为60％、80％、75％、65％、40％、20％，存在显著差异（P〈0．01）。临床随访资料显示：原发灶Prx I蛋白表达阳性组的门静脉癌栓患者（28例），其术后肿瘤首次复发时间（6．3个月）明显晚于Prx I蛋白表达阴性组（12例）患者的时间（3．7个月）。结论肝癌门静脉癌栓组织中存在Prx I蛋白的低度表达；PrxI蛋白的表达与癌栓患者术后复发存在密切关系。Objective To investigate the expression of peroxiredoxin 1 （Prx 1） in hepatocellular carcinoma （HCC） with portal vein tumor thrombosis （PVTT） and to evaluate the relationship between the expressions of Prx 1 and the postoperative recurrence of this disease. Methods Immunohistochemistry and Western blotting were performed to examine the expression of Prx 1 protein in 40 patients with HCC with PVTT. Experiments on Sprague Dawley （SD） rat hepatoma model were further carried out to observe the pathological changes of Prx 1 by immunohistochemistry. Clinical outcomes were analyzed to find a correlation between the recurrence and positive rate of Prx 1. Results The expression level of Prx 1 was significantly up-regulated in primary tumor tissues than in tumor thrombosis samples （P〈0.01）. Immunohistochemistry results showed that the positive rate of Prx 1 in primary tumor tissues were higher than that in tumor thrombosis. Western blotting confirmed a same trend in the level of Prx 1, the average luminosity of the blots were 1534.2 and 735.6, respectively. There was a significant difference in SD rat hepatoma model, the 4, 8, 12, 16, 20 and 24-week positive rates of Prx 1 in liver tumor tissues were 60%, 80%, 75% ,65%, 40% and 25% respectively. Clinical outcomes showed that the time to first postoperative recurrence of Prx 1 in the primary tumor positive group was significantly higher than that in the negative group （6.3 vs 3.7 months, P〈0.01）. Conclusions Prx 1 protein was down-regulated in HCC with PVTT. There was a negative correlation between the expression of Prx 1 and recurrence.

关 键 词：肝细胞癌 门静脉癌栓 PeroxiredoxinI蛋白 复发 

分 类 号：R735.7[医药卫生—肿瘤]