KiSS-1、KiSS-1R及MMP-9表达与肝细胞癌侵袭转移的关系及意义  

Correlation of KiSS-1, KiSS-1R and MMP-9 expression to invasion and metastasis of hepatocellular carcinoma and its significance

作　　者：臧盛兵[1] 刘景丰[2] 王斌[1] 高凌云[1] 陈水平[1] 黄爱民[1] 

机构地区：[1]福建医科大学基础医学院病理学系 福建医科大学肿瘤研究室,福州350004 [2]福建医科大学附属第一医院肝脏外科

出　　处：《中华肝胆外科杂志》2011年第3期223-227,共5页Chinese Journal of Hepatobiliary Surgery

基　　金：基金项目：教育部科学技术研究重点项目（205077）;福建省教育厅科技项目（JA04193,JS06011）

摘　　要：目的检测肿瘤转移抑制基因KiSS-1及其配体KiSS-1R和基质金属蛋白酶-9（MMP-9）在肝细胞癌（HCC）中的表达，探讨KISS-1表达与肝细胞癌侵袭转移的关系及其机制。方法利用逆转录聚合酶链反应（RT—PCR）技术，检测HCC、癌旁肝组织及远癌肝组织中KISS-1、KiSS-1RmRNA的表达情况；应用HCC组织芯片，结合免疫组化及彩色图像分析技术检测了KiSS-1、MMP-9蛋白在HCC芯片中各组织的相对表达量。结果KiSS-1mRNA在HCC中表达明显低于癌旁、远癌肝组织（P〈0．01）。在转移组和临床Ⅲ期HCC中，KiSS-1蛋白的表达明显低于无转移及临床分期I和Ⅱ期HCC（P〈0．01）；KiSS-1在肝内转移灶中的表达量显著低于原发灶（P〈0．01）。在转移组的HCC中，MMP-9表达量显著高于无转移的HCC（P〈0．01）。在HCC组织中，KiSS-1和MMP-9的表达呈负相关性（r=-0．340，P〈0．01）。结论KiSS I和MMP-9的表达失衡与HCC侵袭转移有关，KiSS-1的缺失表达可作为预测HCC转移潜能的有价值的参考指标。Objective To detect the expression of KISS-1 , KiSS-1R and MMP-9 in hepatocellular carcinoma （HCC）. To study the correlation of KISS-1 , KiSS-1R and MMP-9 expression with invasion and metastasis of HCC, and to explore the underlying mechanisms. Methods The expression of KISS-1 , KiSS-IR mRNA in 33 HCC samples, 26 non-neoplastic adjacent liver tissue samples and 13 non-neoplastic distant liver tissue samples were detected by RT-PCR. Tissue chips were constructed by modified manual tools, which contained HCC, non-neoplastic adjacent liver tissues, non-neoplastic distant liver tissues, normal liver tissues and intrahepatic metastasis lesions. The expression of KISS-1 and MMP-9 protein was determined by tissue chips, immunohistochemistry and semi-quantitative image analysis in 150 HCC, 137 non-neoplastic adjacent liver tissue, 98 non-neoplastic distant liver tissues, 16 normal liver tissues and 37 intrahepatic metastasis lesion samples. Results The results of RT-PCR showed that compared with the non-neoplastic adjacent liver tissues and the non-neoplastic distant liver tissues, the expression of KISS-1 mRNA in HCC was significantly lower （P〈0.01）. The expression of KiSS-1R mRNA did not changed in HCC and non-neoplastic liver tissues （P〉0.05）. The expression of KISS-1 protein was lower in HCC with metastasis and in clinical stage III than that in those with non-metastasis, and in clinical stages I and II. It was also higher in the primary than in the metastasis lesions （P〈0.01, respectively）. The expression of MMP-9 was higher in tumors having peplos invasion and metastasis than in those with negative peplos invasion and non-metastasis. It was lower in the primary than the metastasis lesions （P〈0. 01, respectively）. Negative correlation between KISS-1 and MMP-9 expression was found in HCC（r=- 0. 340, P〈0.01）. Conclusions The imbalance between KISS-1 and MMP-9 expression might play an important role in enhancing the invasive and metastatic capacity of HCC. Loss of KISS-1 expression mig

关 键 词：肝肿瘤 KISS-1 KiSS-1R MMP-9 组织芯片 

分 类 号：R735.7[医药卫生—肿瘤]