CD44抗体对视网膜Müller细胞降解透明质酸功能的影响  

Effect of CD44 antibody on hyaluronic acid degradation caused by retinal Mueller cell

作　　者：陆融[1] 乔伟振[2] 

机构地区：[1]南京医科大学附属无锡人民医院眼科,211000 [2]南京医科大学附属无锡人民医院中心实验室,211000

出　　处：《中华眼底病杂志》2011年第2期160-162,共3页Chinese Journal of Ocular Fundus Diseases

基　　金：南京医科大学科技发展基金（09NJMUZ52）

摘　　要：目的观察CD44抗体对视网膜Mueller细胞降解透明质酸（HA）功能的影响。方法培养的猪后极部视网膜Mueller细胞．取第2代细胞用于实验。细胞分为1、2、3组。每组含12×10^3个Mueller细胞。其中1组培养液中未加入其他试剂；2组培养液中加入0．01mg／mlHA；3组培养液中加入0．01mg／mlHA和10μg／ml抗CD44抗体。2、3组细胞和HA、单克隆抗CD44抗体预培养．收集1、2、3组上清液．行HA-底物胶电泳法和类酶链免疫吸附测定（ELISA）法检测。结果HA-底物胶电泳法分析结果显示．1组表现为蓝色背景下的白色细淡的双条带；2组双条带明显增厚，合并成较粗和明亮的脱色条块；3组脱色条块回复为细淡的双条带。类ELISA法检测结果显示．1、2、3组口发光度[A，旧称光密度（OD）]值分别为0．310±0．025、0．093±0．051、0．025±0．069。2组A值较1组A值明显降低，与1组A值比较．差异有统计学意义（t=28．1．P〈0．01）；3组A值与1组A值比较．差异无统计学意义（t=4．92，P〉0．05），与2组A值比较．差异有统计学意义（t=26．9，P〈0．01）。结论Mueller细胞与HA的相互作用可加强细胞降解HA的功能，CD44抗体可降低这种加强作用。Objective To observe the effect of CD44 antibody on the hyaluronic acid （HA） degradation mediated by retinal Mueller cell Methods Pig retinal Mueller cells from the posterior pole （2nd generation） were cultured in three different medium： without HA （group 1 ）, 0.01 mg/ml HA （group 2 ）. 10 μg/ml HA and CD44 antibody （group 3）. The cells in the group 2 and 3 were pre-cultured with HA and CD44 antibody, and the supernatant was collected. HA substrate gel electrophoresis was performed for HA degradation, while ELISA-Iike method was performed for HA binding protein. Results HA substrate gel electrophoresis showed white light double-band on blue background in groups 1 and 3, thicker double band or bright de-colored blocks in group 2. ELISA-Iike method showed that the absorb＇ance （A） value of groups 1, 2 and 3 were 0.310-1-0.025, 0.093±0.051, 0.025±0.069 respectively. The A value of group 2 was obviously lower than that of group 1 （t=28. 1, P〈0.01）, theA value of group 3 was significanlly higher than that of group 2 （t=26.9, P〈70.01）, but was the same as group 1 （t=4.92, P±0.05）. Conclusion CD44 antibody can inhibit the interaction between Mueller cells and HA, and thus reduce the HA degradation.

关 键 词：细胞黏附分子/拮抗剂和抑制剂 透明质酸/代谢 MUELLER细胞 

分 类 号：R774.1[医药卫生—眼科]