静脉注射α-晶体蛋白对视网膜神经节细胞的保护作用及对重要脏器的影响  

Effects of intravenous injection of α-crystallin on retinal ganglion cells and some important organs

作　　者：于嘉[1] 王一[1] 

机构地区：[1]第三军医大学附属西南医院眼科西南眼科医院,重庆400038

出　　处：《中华眼底病杂志》2011年第2期163-166,共4页Chinese Journal of Ocular Fundus Diseases

摘　　要：目的观察静脉注射α-晶体蛋白对Long Evans大鼠视神经损伤后视网膜神经节细胞（RGC）存活的保护作用及对重要脏器的影响。方法23只Long Evans大鼠用于本实验。荧光金经双侧上丘及外侧膝状体逆行标记RGC，7d后制作视神经钳夹伤模型。3只作为正常对照组，其余20只随机分为生理盐水对照组以及1×10^-2g／L、1×10^-1g／L、1g／L α-晶体蛋白组，每组5只大鼠。视神经钳夹伤后经尾静脉分别注射1．25ml的等渗生理盐水及3个浓度的α-晶体蛋白，每2天重复注射1次，共7次。2周后对实验大鼠行RGC计数，并对肝、肾、脑、脾、肺等脏器进行病理观察。结果正常对照组RGC计数（2074±150）个／mm^2，视神经钳夹伤后2周时RGC计数，生理盐水对照组（85±15）个／mm^2，1×10^-2g／Lα-晶体蛋白组（124±26）个／mm^2，1×10^-1g／L α-晶体蛋白组（128±31）个／mm^2，1g／L α-晶体蛋白组（164±20）个／mm^2。正常对照组RGC计数显著高于其他组，3个浓度α-晶体蛋白组存活的RGC数均显著高于生理盐水对照组（F=18．660，P〈0．01）。各组肝、肾、脑、脾、肺病理观察未见充血、肿大、炎症等病理改变。结论静脉注射α-晶体蛋白对视神经损伤后RGC存活具有一定保护作用，所用α-晶体蛋白浓度对重要脏器无病理性影响。Objective To investigate the effects of intravenous injection of α -crystallin on retinal ganglion cells （RGC） and some important organs of the Long Evans rats. Methods RGC were retrograde labeled by fluorogold through bilateral superior colliculus and lateral geniculate body for seven days before optic nerve crush injury. Twenty-three Long Evans rats were used for this study, including three rats of normal control group and 20 rats of experimental group. Twenty rats were randomly divided into saline control group and three α-crystallin injection groups, which received tail vein injection of 1.25 ml isotonic saline and three different concentrations （1 × 10^-2 , 1 × 10^-1 and 1 g/L） of α-crystallin respectively, once every two days and totally seven times. After two weeks, the labeled RGC were counted, and the pathological changes on liver, kidney, brain, spleen and the lungs were investigated. Results Compared with the normal control group, although the number of RGC markedly decreased after two weeks of optic nerve crush injury in every group, the number of RGC in α-crystallin-treated groups was more than those in the saline control group. There were 2074 ± 150 RGC per mm2 in normal control group, 85±V 15 RGC per mm2 in saline control group, 124±26 RGC per mm2 in 1 × 10-2 g/L α-crystallin group, 128± 31 RGC per mm2 in 1×10^-1 g/L α-crystallin group, 164±20 RGC per mm2 in 1 g/L α-crystallin group （F： 18. 660, P〈0.01）. No congestion, swelling, inflammation and other pathological changes were found in liver, kidney, brain, spleen and lung. Conclusions Intravenous injection of α- crystallin protein has protective effects on RGC after the optic nerve crush injury, and no significant effects on important organs.

关 键 词：α晶体蛋白质类/药物作用 α晶体蛋白质类/投药和剂量 动物实验 

分 类 号：R774.1[医药卫生—眼科]