15d-PGJ_2减弱香烟提取物抑制人脐静脉内皮细胞迁移  

15d-PGJ_2 attenuates CSE-induced inhibition of HUVECs migration

作　　者：李炳蔚[1] 苑晓晨[1] 李宏伟[1] 修瑞娟[1] 

机构地区：[1]中国医学科学院北京协和医学院微循环研究所,北京100005

出　　处：《基础医学与临床》2011年第4期394-399,共6页Basic and Clinical Medicine

基　　金：中国-瑞典合作研究基金(IM2008RJ2)

摘　　要：目的探讨15-脱氧-Δ12,14-前列腺素J2(15d-PGJ2)对香烟提取物(CSE)抑制人脐静脉内皮细胞(HUVECs)迁移的保护作用。方法 "划痕法"检测HUVECs的迁移。毛细管样成管实验检测HUVECs的血管新生。Western blot检测occludin蛋白表达。结果与对照组相比,CSE显著抑制HUVECs迁移,使单个视野划痕内细胞数由629±28降低至364±17(P<0.01),15d-PGJ2干预可使细胞数回升至546±20(P<0.01)。CSE抑制HUVECs血管新生,15d-PGJ2可逆转CSE对HUVECs血管新生的抑制。与对照组相比,CSE使HUVECs occludin蛋白表达量由0.37±0.04降至0.12±0.03(P<0.01)。15d-PGJ2干预可使其回调至0.28±0.04(P<0.01),但仍低于对照组(P<0.05)。结论 CSE对HUVEVs迁移的抑制作用可能是通过下调occludin蛋白的表达实现的。15d-PGJ2减弱CSE下调occludin表达而逆转CSE对HUVECs迁移的抑制。Objective To explore the impact of 15-deoxy-Δ12,14-prostaglandin J2（15d-PGJ2） on cigarette smoke extract（CSE）-induced change of human umbilical vein endothelial cells（HUVECs） migration.Methods To determine the migration and angiogenesis of HUVECs with＂scratch assay＂ and capillary-like tube formation assay.To observe the expression of occludin with Western blot.Results CSE significantly inhibited the migration of HUVECs.Cell number of wound closure.15d-PGJ2 treatment increased the cell number of wound closure from 364±17 in CSE group to 546±20 in 15d-PGJ2＋CSE group.CSE inhibited the angiogenesis of HUVECs.15d-PGJ2 attenuated CSE-induced inhibition of HUVECs angiogenesis.The expression of occludin decreased from 0.37±0.04 in control to 0.12±0.03 in CSE group and increased to 0.28±0.04 in 15d-PGJ2＋CSE group.ConclusionCSE may inhibit the migration of HUVECs through down-regulation of occludin.15d-PGJ2 may attenuate the change of occludin to rescue the HUVECs migration inhibited by CSE.

关 键 词：香烟提取物 内皮细胞迁移 15-脱氧-前列腺素J2 

分 类 号：R363.2[医药卫生—病理学] R364.5[医药卫生—基础医学]