磁流体热疗对体外人肺癌细胞A549的影响  

Effects of Magnetic Fluid Hyperthermia Induced by An Alternative Magnetic Field on Human Carcinoma A549 Cell in vitro

作　　者：王国卿[1] 李浒[1] 胡润磊[1] 柯贤福[1] 魏东山[1] 孟文[1] 

机构地区：[1]杭州市第一人民医院胸外科,杭州310006

出　　处：《中国肺癌杂志》2011年第3期181-186,共6页Chinese Journal of Lung Cancer

基　　金：浙江省医药卫生科学研究基金(No.2009A163);杭州市科技发展计划项目(No.20080333B02);杭州市医药卫生科技计划(No.2007B001)资助~~

摘　　要：背景与目的磁流体热疗是将纳米技术和热疗相结合的一种新兴热疗方法,具有高度的靶向性和特异性,已成为目前研究的热点。本研究旨在探讨Fe3O4纳米磁流体热疗在体外对人肺癌A549细胞的影响。方法在体外将不同浓度的Fe3O4纳米磁流体和人肺癌A549细胞共培养,在交变磁场中作用30min,采用M法细胞计数、流式细胞术、倒置显微镜、透射电镜等方法观察磁流体热疗对人肺癌A549细胞活细胞数的光密度值、杀伤率、细胞形态、细胞周期及凋亡情况的影响。结果磁流体热疗后人肺癌A549细胞增殖受到明显抑制,活细胞数的光密度值下降;杀伤率(cytotoxityindex,CI)增加;细胞凋亡率逐渐增强;细胞周期停滞于S期,S期细胞和G2期增加,与磁流体浓度呈明显依赖关系。电镜观察磁流体热疗后的肺癌细胞呈凋亡样改变,高温时呈坏死样改变。结论磁流体热疗能明显抑制人肺癌A549细胞的增殖,诱导凋亡,阻滞细胞于S期。Background and objective Magnetic fluid hyperthermia（MFH） is a method of heat therapy using nanometer techniques and hyperthermia.It has the advantage of high specificity of targeting.The aim of this study is to detect the effects of MFH induced by an alternating magnetic field on human being carcinoma A549 cells in vitro.Methods A human adenocarcinoma cell line A549 was cultured with various concentrations of ferroferric oxide（Fe3O4） magnetic fluid（1.5-6.0） mg/mL and exposed to an alternative magnetic field（AMF） for 30 min.And then the optical density（OD） of viable cell,cytotocixity index,growth curve of cells,morphologic changes of cell,cell cycle and aposptosis were measured.Results The proliferation of the A549 cells were remarkably inhibited,the OD value of viable cells decreased and cytotox-ity index（CI） increased;Apoptosis of the A549 cells were observed to have cell shrinkage,chromatin condensation,margination,unclear fragmentation and intact cell membrane by light and electron microscopy;The cells were inhibited in the stage S.Conclusion MFH induced by AMF could inhibit the proliferation,which promotes apoptosis and arrest at S stage of the A549 cells.

关 键 词：肺肿瘤 凋亡 体外 

分 类 号：R734.2[医药卫生—肿瘤]