成釉细胞瘤中p-AKT、PI3K、PDGF及其受体的表达意义  

Expression and significance of p-AKT,PI3K,PDGF and its receptor in ameloblastomas

作　　者：尹晓东[1] 王德明[1] 焦晓辉[1] 

机构地区：[1]哈尔滨医科大学附属口腔医学院口腔颌面外科,黑龙江哈尔滨150001

出　　处：《哈尔滨医科大学学报》2011年第1期39-42,45,共5页Journal of Harbin Medical University

基　　金：黑龙江省青年科学基金项目(QC2009C91);黑龙江省卫生厅医学科研课题资助项目(2006045);哈尔滨医科大学附属第一医院科研基金资助项目(2007116)

摘　　要：目的探讨血小板衍生生长因子-A(PDGF-A)及其受体(PDGFR-α)3、羧基磷脂酰肌醇激酶(PI3K)和磷酸化蛋白激酶B(p-AKT)在成釉细胞瘤(AB)中的表达及意义。方法应用免疫组化法检测9例牙胚、36例成釉细胞瘤中上述4种蛋白的表达。结果 PDGF-A表达在牙胚与滤泡型AB、滤泡型AB与丛状型AB之间比较有显著差异(P<0.05);PDGFR-α表达在棘皮瘤型AB、颗粒细胞型AB和无细胞变异型AB之间比较有显著差异(P<0.05);PI3K表达在牙胚组与丛状型AB和无细胞变异型AB比较、滤泡型AB与丛状型AB比较差异均有显著性(P<0.05);p-AKT表达在牙胚组分别与AB、丛状型AB和无细胞变异型AB比较均有显著差异(P<0.05)。结论牙源性组织中PDGF-A/PDGFR-α功能的发挥可能通过PI3K/AKT信号传导通路的介导实现。PDGF及其受体的高表达及PI3K/AKT信号传导通路异常活化与成釉细胞瘤的发生、发展有关。Objective To investigate the expression and its significance of platelet-derived growth factor-A（PDGF-A） and its receptor（PDGFR-α）,PI3K and p-AKT in ameloblastomas（ABs）.Methods The expressions of above-mentioned 4 proteins in 9 tooth germs and 36 Abs were detected by using immunohistochemistry（IHC）.Results PDGF-A expression was significantly different between tooth germs and plexiform ABs,plexiform ABs and follicular ABs（P〈0.05）;PDGFR-α expression was significantly different among acanthomatous subtype,granular subtype and non-cellular variation ABs（P〈0.05）;PI3K expression was significantly different among tooth germs,plexiform ABs and non-cellular variation ABs,and between plexiform and follicular ABs（P〈0.05）;p-AKT expression was significantly different between tooth germs and ABs,plexiform ABs and non-cellular variation ABs respectively（P〈0.05）.Conclusion Function exertion of PDGF-A/PDGFR-α in odontogenic tissues could be completed which was mediated by PI3K/AKT signal transduc-tion pathway.Genesis and development of AB is correlated with high expression of PDGF and its receptor,and abnomal activation of PI3K/AKT signal transduction pathway.

关 键 词：成釉细胞瘤 3羧基磷脂酰肌醇激酶 蛋白激酶B 血小板衍生生长因子 

分 类 号：R-332[医药卫生] R739.8